Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Oxidative demethylation of 1-meA damaged DNA By ALKBH3
Oxidative demethylation of 3-meC damaged DNA By ALKBH3
Oxidative dealkylation of 1-EtA damaged DNA by ABH3
ALKBH3 associated with ASCC1:ASCC2:ASCC3 binds alkylated dsDNA containing 3-meC
ALKBH3 associated with ASCC1:ASCC2:ASCC3 binds alkylated dsDNA containing 1-meA
ALKBH3 in complex with ASCC1:ASCC2:ASCC3 binds alkylated DNA containing 1-etA
Reversal of DNA alkylation damage by two human dioxygenases.
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ALKBH3 removes m1A and m3C by direct damage reversal.
"Both enzymes remove 1-methyladenine and 3-methylcytosine from methylated polynucleotides in an alpha-ketoglutarate-dependent reaction, and act by direct damage reversal with the regeneration of the unsubstituted bases."
Human and bacterial oxidative demethylases repair alkylation damage in both RNA and DNA.
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ALKBH3 also repairs RNA.
"AlkB and hABH3, but not hABH2, also repair RNA"
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ALKBH3 prefers single-stranded nucleic acids.
"Whereas AlkB and hABH3 prefer single-stranded nucleic acids, hABH2 acts more efficiently on double-stranded DNA."
Repair of methylation damage in DNA and RNA by mammalian AlkB homologues.
Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage.
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The ALKBH3 structure shows the catalytic iron centre.
"The hABH3 structure reveals the beta-strand jelly-roll fold that coordinates a catalytically active iron centre by a conserved His1-X-Asp/Glu-X(n)-His2 motif."
Expression and sub-cellular localization of human ABH family molecules.
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Tagged ALKBH3 is nuclear and cytoplasmic.
"EmGFP-hABH1, 3, 4, 6 and 7 were localized in both the cytoplasm and nuclei."
Mechanistic insight into the recognition of single-stranded and double-stranded DNA substrates by ABH2 and ABH3.
DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation.
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ALKBH3 associates with the ASCC complex.
"We report the purification of the ALKBH3 complex and demonstrate its association with the activating signal cointegrator complex (ASCC)."
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ASCC3 unwinds DNA for ALKBH3.
"ASCC3, the largest subunit of ASCC, encodes a 3'-5' DNA helicase, whose activity is crucial for the generation of single-stranded DNA upon which ALKBH3 preferentially functions for dealkylation."
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Loss of ALKBH3 raises m3C and reduces proliferation.
"loss of ALKBH3 or ASCC3 leads to increased 3-methylcytosine and reduced cell proliferation"
The dynamic N(1)-methyladenosine methylome in eukaryotic messenger RNA.
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ALKBH3 lowers mRNA m1A.
"overexpression of wild-type ALKBH3, but not of an inactive mutant, reduced m1A levels in mRNA"
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m1A correlates with translation.
"strongly indicating a functional role for m(1)A in promoting translation of methylated mRNA."
Transcriptome-wide mapping reveals reversible and dynamic N(1)-methyladenosine methylome.
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m1A in mRNA is reversible by ALKBH3.
"Moreover, m(1)A in mRNA is reversible by ALKBH3, a known DNA/RNA demethylase."
A ubiquitin-dependent signalling axis specific for ALKBH-mediated DNA dealkylation repair.
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ALKBH3 functions with the ASCC helicase complex.
"Previous studies established that the dealkylating enzyme, ALKBH3, functions in concert with the ASCC helicase complex"
A proteome-scale map of the human interactome network.
An interactome perturbation framework prioritizes damaging missense mutations for developmental disorders.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
Affinage mechanistic annotation for ALKBH3 (human)
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Affinage summarizes ALKBH3 as a single-stranded DNA/RNA m1A/m3C demethylase.
"ALKBH3 is a single-stranded nucleic acid demethylase that reverses N1-methyladenine/N1-methyladenosine (m1A) and N3-methylcytosine/N3-methylcytidine (m3C) lesions in both DNA and RNA"
UniProtKB entry Q96Q83 (ALKB3_HUMAN)