Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Zebrafish contains two pax6 genes involved in eye development.
Stereospecificity and PAX6 function direct Hoxd4 neural enhancer activity along the antero-posterior axis.
Subfunctionalization of duplicated zebrafish pax6 genes by cis-regulatory divergence.
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Pax6a and Pax6b activate paired-domain and homeodomain reporter targets at equivalent levels.
"Activation by the two wild type co-orthologues through the P3 homeodomain target [45] (Figure 1D), or the CD19 paired domain target [46] (Figure 1E), was not significantly different, in contrast to the original observations by Nornes et al [43]."
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Only pax6b is expressed in the pancreas; pax6a is not induced there in sunrise mutants.
"only pax6b is expressed in the pancreas isolated from 6 month old wild type and sri/sri fish, while eyes from the same individuals express both pax6a and pax6b"
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pax6a has the broader neural expression domain and lacks pancreatic expression.
"pax6a is expressed in the lens and retina, as well as more widely in the developing telencephalon, diencephalon, hind brain, and spinal cord, although not in developing pancreas [49]."
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The pax6a locus retains most ancestral cis-regulatory elements.
"Zebrafish LG25 retains pax6a synteny with wt1a and with the majority of pax6 cis-regulatory elements, but rcn1 and elp4 coding exons have been lost."
Expression of zebrafish pax6b in pancreas is regulated by two enhancers containing highly conserved cis-elements bound by PDX1, PBX and PREP factors.
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pax6a enhancers A and C are inactive in pancreas owing to PDX1-site divergence.
"In constrast, regions A and C of the zebrafish pax6a gene are not active in the pancreas, this difference being attributable to sequence divergences within two cis-elements binding the pancreatic homeoprotein PDX1."
Pax6a and Pax6b are required at different points in neuronal progenitor cell proliferation during zebrafish photoreceptor regeneration.
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Pax6a is required for later divisions of regenerating retinal neuronal progenitors.
"In contrast, the paralogous Pax6a protein was required for later neuronal progenitor cell divisions, which maximized the number of neuronal progenitors."
The Pax6b homeodomain is dispensable for pancreatic endocrine cell differentiation in zebrafish.
Sequencing of Pax6 loci from the elephant shark reveals a family of Pax6 genes in vertebrate genomes, forged by ancient duplications and divergences.
Pax6 regulates the formation of the habenular nuclei by controlling the temporospatial expression of Shh in the diencephalon in vertebrates.
Evolution of the vertebrate Pax4/6 class of genes with focus on its novel member, the Pax10 gene.
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pax6a and pax6b arose in the teleost-specific genome duplication.
"By conducting rigorous molecular phylogenetic analyses and considering conserved synteny, we demonstrate that Pax4, -6, and -10 originated in the 2R-WGD, and that the two pax6 genes of teleost fishes (pax6a and pax6b) were duplicated in the TSGD."
Molecular description of eye defects in the zebrafish Pax6b mutant, sunrise, reveals a Pax6b-dependent genetic network in the developing anterior chamber.
Habenular Neurogenesis in Zebrafish Is Regulated by a Hedgehog, Pax6 Proneural Gene Cascade.
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Habenular neurogenesis requires pax6a but not pax6b.
"Somewhat surprisingly, on the other hand, morpholino knock-down of pax6a alone abrogated the expression of both markers (Fig 2C and 2G); pax6a morphant/pax6bsa86 mutant embryos behaved the same as pax6a morphants alone with respect to these markers (Fig 2D and 2H)."
Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves.
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pax6a/b dosage controls periocular neural crest guidance and anterior segment formation.
"Taken together, our data are consistent with a gene number dependent effect of pax6a/b on the migration of the two distinct waves of NC cells."
Medaka (Oryzias latipes) Olpax6.2 acquires maternal inheritance and germ cells expression, but functionally degenerate in the eye.