Mycobacterium tuberculosis H(2)S Functions as a Sink to Modulate Central Metabolism, Bioenergetics, and Drug Susceptibility.
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Identified Rv3684 (Cds1) as an H2S-producing enzyme in Mtb
"We identified Rv3684 (Cds1) as an H2S-producing enzyme in Mtb and show that cds1 disruption reduces, but does not eliminate, H2S production, suggesting the involvement of multiple genes in H2S production"
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Demonstrated cysteine desulfhydrase activity producing H2S and pyruvate
"Identification of the enzymatic products indicates that Cds1 is a cysteine desulfhydrase that generates H2S and pyruvate from Cys."
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Showed PLP-dependence through AOAA inhibition
"We showed that this activity is PLP-dependent, is inhibited by AOAA and not PAG, and uses Cys as a sulfur source."
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Kinetic parameters KM = 11.26 mM, kcat = 78.71 sec(-1)
"we determined the Km of Rv3684 to be 11.26 ± 0.75 mM with a kcat of 78.71 ± 12.72 S−1"
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Delta-cds1 reduces H2S production and basal oxygen consumption
"cds1 disruption reduces, but does not eliminate, H2S production"
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H2S stimulates respiration via cytochrome bd
"maintains bioenergetic homeostasis by stimulating respiration primarily via cytochrome bd"
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H2S modulates redox homeostasis (EGT, MSH levels)
"Lastly, our data demonstrate that Cds1-generated H2S modulates the levels of the major redox couples, EGT and MSH."
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Cds1 likely eliminates toxic levels of cysteine
"Cds1 can mitigate toxic levels of Cys by converting excess Cys into H2S"
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Part of Rv3682-Rv3683-cds1 operon
"cds1 is in an operon with rv3682 (ponA2) and rv3683"