Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
A human microprotein that interacts with the mRNA decapping complex.
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NoBody binds EDC4 directly (pull-down, photo-crosslinking); residues 22-41 are necessary and sufficient, with L30 and W34 essential.
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NoBody levels are anticorrelated with P-body number; overexpression disperses P-bodies and knockdown increases them.
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NoBody levels change steady-state abundance of one NMD substrate; decay rate not measured.
The NBDY Microprotein Regulates Cellular RNA Decapping.
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Endogenous NBDY binds the EDC4 WD40 domain and localizes to P-bodies; NBDY KO triples P-bodies and alters >1400 RNA half-lives bidirectionally.
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NBDY does not alter in vitro decapping activity; its effect on DCP2 substrates requires the EDC4 interaction and correlates with 5' UTR length.
Phosphorylation of a Human Microprotein Promotes Dissociation of Biomolecular Condensates.
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NBDY forms liquid condensates with RNA in vitro; phosphorylation (PKC) dissolves them.
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NBDY is phosphorylated at T40 in mitosis (CDK-dependent) and S61 after EGF (PKC); phosphosite mutants block P-body loss under these conditions.
Assembly and regulation of the mammalian mRNA processing body.
EDC4 C-terminal domain scaffolds P-body assembly and links P-body dynamics to p53-mediated tumor suppression.
ATP Acts as a Hydrotrope to Regulate the Phase Separation of NBDY Clusters.