Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Combined Automated Annotation using Multiple IEA Methods
Fatty acid biosynthesis in Pseudomonas aeruginosa is initiated by the FabY class of β-ketoacyl acyl carrier protein synthases.
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PA5174 alone complements FabH depletion and fabH deletion in E. coli.
"PA5174 is thus by itself sufficient to complement FabH and restore initiation of FAS"
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Purified FabY condenses acetyl-CoA with malonyl-ACP at a rate comparable to E. coli FabH and prefers C2-C6 acyl-CoA acceptors.
"PA5174 utilized short-chain acyl-CoA as substrates, including acetyl-CoA, butyryl-CoA, and hexanoyl-CoA, but did not use any of the longer-chain acyl-CoA substrates"
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ΔfabY specifically reduces de novo fatty acid synthesis flux.
"However, the larger decrease in the rate of label incorporation for FAS in comparison to RNA indicates particular attenuation specific to FAS"
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Defines the FabY class of KASI/II-domain initiating condensing enzymes.
"We propose distinguishing β-ketoacyl-ACP synthases with KASI/II domains and a preference for short-chain acyl-CoA acceptor substrates as the new FabY class of condensing enzymes"
Deletion of the β-acetoacetyl synthase FabY in Pseudomonas aeruginosa induces hypoacylation of lipopolysaccharide and increases antimicrobial susceptibility.
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ΔfabY lipid A mostly lacks one secondary lauroyl chain, attributed to limited C12:0-ACP supply.
"The majority of lipid A species in the Δ fabY mutant lacked a single secondary lauroyl group, resulting in hypoacylated lipid A."
Pseudomonas aeruginosa directly shunts β-oxidation degradation intermediates into de novo fatty acid biosynthesis.
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Exogenous fatty acids bypass FabY via the KASIII enzyme PA3286, which condenses C8-CoA with malonyl-ACP.
"PA3286 in P. aeruginosa PAO1 intercepts C(8)-CoA by condensation with malonyl-ACP"
Evolutionary origins of the multienzyme architecture of giant fungal fatty acid synthase.
UniProt record for Pseudomonas aeruginosa PAO1 fabY (Q9HU15)