GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

PMID:22898498

Two novel RING-type ubiquitin ligases, RGLG3 and RGLG4, are essential for jasmonate-mediated responses in Arabidopsis.

Both RGLG3 and RGLG4 possessed ubiquitin ligase activities and were widely distributed in Arabidopsis tissues. AtRGLG4 (Q9SAL0) is the closest characterized ortholog of OsRGLG4/Q6YYC5.

PMID:27497447

The Arabidopsis Iron-Sulfur Protein GRXS17 is a Target of the Ubiquitin E3 Ligases RGLG3 and RGLG4.

Identified the cognate E2 enzyme UBC30 and substrate GRXS17 for AtRGLG3/4, demonstrating both in vitro ubiquitination and in vivo substrate degradation.

PMID:37177781

The E3 ubiquitin ligase OsRGLG5 targeted by the Magnaporthe oryzae effector AvrPi9 confers basal resistance against rice blast.

file:ORYSJ/Q6YYC5/Q6YYC5-hypotheses/core-function-1-go-0061630/openscientist.md

OpenScientist hypothesis investigation - E3 ubiquitin ligase activity as core function

GO:0061630 strongly supported. Recommend upgrading from GO:0004842 (parent E2+E3 term) to GO:0061630 (E3-specific term). Multiple IDA-level demonstrations in closest ortholog AtRGLG4 and family-wide conservation.
Q6YYC5 has a myristoylation-compatible N-terminus (MG motif) suggesting possible plasma membrane association, qualifying CC annotations but not core MF.

file:ORYSJ/Q6YYC5/Q6YYC5-deep-research-falcon.md

Deep research report for Q6YYC5 Os08g0135400 RGLG family E3 ligase