Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
CIA30 complex I assembly factor: a candidate for human complex I deficiency?
-
Human CIA30 is homologous to a Neurospora complex-I assembly factor that is absent from the mature complex.
"These proteins are not constituent parts of the mature complex but are associated with smaller intermediate complexes of the assembly process and have a chaperone-like function."
Human mitochondrial complex I assembly is mediated by NDUFAF1.
-
NDUFAF1 is mitochondrial and is required for complex-I assembly.
"Here, we demonstrate that NDUFAF1 is a mitochondrial protein that is involved in the complex I assembly process."
Cytosolic signaling protein Ecsit also localizes to mitochondria where it interacts with chaperone NDUFAF1 and functions in complex I assembly.
Human CIA30 is involved in the early assembly of mitochondrial complex I and mutations in its gene cause disease.
-
NDUFAF1 associates transiently with newly translated mitochondrial complex-I subunits during early assembly.
"human complex I assembly factor CIA30 (complex I intermediate associated protein) associates with newly translated mtDNA-encoded complex I subunits at early stages in their assembly before dissociating at a later stage."
Acyl-CoA dehydrogenase 9 is required for the biogenesis of oxidative phosphorylation complex I.
A reference map of the human binary protein interactome.
Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I.
-
NDUFAF1 is a component of the MCIA complex required to build the ND2 module.
"The mitochondrial complex I intermediate assembly (MCIA) complex, containing assembly factors NDUFAF1, ECSIT, ACAD9, and TMEM126B, is required for building the intermediate ND2-module."
Assembly of The Mitochondrial Complex I Assembly Complex Suggests a Regulatory Role for Deflavination.
TMEM70 and TMEM242 help to assemble the rotor ring of human ATP synthase and interact with assembly factors for complex I.
Molecular mechanism of interactions between ACAD9 and binding partners in mitochondrial respiratory complex I assembly.
-
ACAD9, ECSIT, and NDUFAF1 form a stable ternary MCIA core complex.
"ACAD9/ECSIT/NDUFAF1 ternary complex is soluble and highly stable."
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex
The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I
ND4, ND5 bind the 550kDa complex to form the 815kDa complex
COA1:MT-ND2, TMEM186:MT-ND3, MT-ND6, NDUFB6 bind the MCIA complex to form a 370kDa subcomplex
The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex