Gene Ontology annotation through association of InterPro records with GO terms
Combined Automated Annotation using Multiple Sources
The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter.
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CHL1 was identified via chlorate (herbicide) sensitivity and encodes a nitrate-inducible nitrate transporter.
"The herbicide sensitivity gene CHL1 of Arabidopsis encodes a nitrate-inducible nitrate transporter"
The Arabidopsis CHL1 protein plays a major role in high-affinity nitrate uptake.
CHL1 is a dual-affinity nitrate transporter of Arabidopsis involved in multiple phases of nitrate uptake.
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CHL1 is a dual-affinity nitrate transporter with high-affinity (Km ~49 uM) and low-affinity (Km ~4 mM) phases.
"CHL1 is a dual-affinity nitrate transporter of Arabidopsis involved in multiple phases of nitrate uptake"
The nitrate transporter AtNRT1.1 (CHL1) functions in stomatal opening and contributes to drought susceptibility in Arabidopsis.
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CHL1 functions in stomatal opening; chl1 mutants show reduced transpiration and enhanced drought tolerance.
"The nitrate transporter AtNRT1.1 (CHL1) functions in stomatal opening and contributes to drought susceptibility in Arabidopsis"
Transcript profiling in the chl1-5 mutant of Arabidopsis reveals a role of the nitrate transporter NRT1.1 in the regulation of another nitrate transporter, NRT2.1.
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NRT1.1/CHL1 regulates expression of the nitrate transporter NRT2.1.
"a role of the nitrate transporter NRT1.1 in the regulation of another nitrate transporter, NRT2.1"
The Arabidopsis NRT1.1 transporter participates in the signaling pathway triggering root colonization of nitrate-rich patches.
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NRT1.1/CHL1 participates in nitrate signaling triggering root colonization of nitrate-rich patches (response to nitrate).
"The Arabidopsis NRT1.1 transporter participates in the signaling pathway triggering root colonization of nitrate-rich patches"
CHL1 functions as a nitrate sensor in plants.
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CHL1 functions as a nitrate sensor (transceptor); the sensing function is separable from uptake activity and is controlled by CIPK23 phosphorylation of Thr101.
"CHL1 functions as a nitrate sensor in plants"
Nitrate-regulated auxin transport by NRT1.1 defines a mechanism for nutrient sensing in plants.
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NRT1.1/CHL1 facilitates auxin transport in a nitrate-regulated manner, linking nitrate availability to root development.
"Nitrate-regulated auxin transport by NRT1.1 defines a mechanism for nutrient sensing in plants"
Arabidopsis NRT1.1 is a bidirectional transporter involved in root-to-shoot nitrate translocation.
Crystal structure of the plant dual-affinity nitrate transporter NRT1.1.
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The crystal structure of NRT1.1/CHL1 reveals an MFS-fold nitrate transporter; mutagenesis defines the nitrate-binding pocket and proton-coupling machinery.
"Crystal structure of the plant dual-affinity nitrate transporter NRT1.1"
Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1.
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Structural and mutagenesis analysis (Arg45, Thr101, Lys164, His356) defines the molecular basis of nitrate uptake by NRT1.1/CHL1.
"Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1"
NRT1.1 Regulates Nitrate Allocation and Cadmium Tolerance in Arabidopsis.
Phosphorylation-Mediated Dynamics of Nitrate Transceptor NRT1.1 Regulate Auxin Flux and Nitrate Signaling in Lateral Root Growth.
Falcon (Edison Scientific) deep research report: Arabidopsis thaliana NPF6.3 (CHL1/NRT1.1; At1g12110; UniProt Q05085) functional annotation
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"NPF6.3/CHL1/NRT1.1 is widely regarded as a **dual-affinity** nitrate transporter with **biphasic kinetics**, enabling transport across a wide external nitrate range."
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"A core concept in nitrate biology is that NPF6.3 acts as a **transceptor**—a transporter with receptor-like signaling capability—helping initiate the **primary nitrate response (PNR)** and modulating expression of other nitrate transport genes (e.g., **NRT2.1**) and developmental programs."
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"NPF6.3 is described as a **proton-coupled nitrate symporter**."
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"A central and well-supported mechanism is that **Thr101** serves as an affinity-mode switch: phosphorylation favors high-affinity transport, whereas dephosphorylation favors low-affinity transport."
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"Nitrate-induced Ca2+ signals are perceived by **CBL1/CBL9**, activating **CIPK23**, which phosphorylates NRT1.1 at Thr101 and shifts apparent affinity from **Km ~4 mM** to **Km ~40 µM**."
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"localizes to the **plasma membrane** of **root epidermal** and **vascular** cells, with additional localization reported in **guard cells**"
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"NRT1.1 facilitates basipetal auxin transport and negatively regulates auxin biosynthesis/transport-related genes (e.g., TAR2, LAX3), thereby removing auxin from lateral root primordia under low nitrate and inhibiting lateral root growth"
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"NRT1.1/NPF6.3 is described as regulating expression of NRT2.1 and other primary nitrate-response genes"
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"Across sources, nitrate (NO3−) is the core substrate, and NPF6.3 contributes substantially to nitrate uptake"
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"The CHL1 name is linked to chlorate resistance screens; chlorate is a nitrate analog used in genetics, supporting the nitrate-related substrate recognition and historical discovery context."
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"AtNPF6.3 has been estimated to contribute roughly 10–80% of whole-plant nitrate uptake."