Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Isolation and characterization of the human melanin-concentrating hormone gene and a variant gene.
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The variant MCH gene shares coding sequence with the C-terminal part of the MCH prohormone but not the N-terminal part.
"Cloning from three genomic libraries and sequencing of one class of hMCH genomic DNA reveal high similarity between coding regions and the C-terminal part of the hMCH prohormone"
Assignment of the human pro-melanin-concentrating hormone gene (PMCH) to chromosome 12q23-q24 and two variant genes (PMCH1 and PMCHL2) to chromosome 5p14 and 5q12-q13.
Antisense expression of the human pro-melanin-concentrating hormone genes.
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Brain transcripts from the variant locus are antisense, and there is no evidence that an MCH precursor is made from the pMCHL loci.
"Thus, there is no evidence that the MCH peptide-precursor molecule is produced in the brain by the human-variant pMCHL loci."
Structure and expression of the variant melanin-concentrating hormone genes: only PMCHL1 is transcribed in the developing human brain and encodes a putative protein.
Birth of two chimeric genes in the Hominidae lineage.
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PMCHL1 is a chimeric gene created by retrotransposition of antisense MCH mRNA.
"the PMCHL1 gene was created near 25 million years ago (Ma) by a complex mechanism of exon shuffling through retrotransposition of an antisense MCH messenger RNA coupled to de novo creation of splice sites"
Primate-specific spliced PMCHL RNAs are non-protein coding in human and macaque tissues.
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No protein product of PMCHL ORFs is detectable in tissues expressing PMCHL RNA.
"This strongly suggests that these putative proteins are not translated in vivo in the human and macaque tissues that we tested."
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PMCHL transcripts are proposed to function as mRNA-like noncoding RNAs.
"It is tempting to propose that these PMCHL transcripts work mainly as an mRNA-like non-protein coding RNA (ncRNA)."
Pairwise comparison of PMCHL1 (Q16048) with parent pro-MCH (PMCH, P20382)