NDE1 review notes

2026-09-27 initial review (claude-code)

Sources: UniProt Q9NXR1, GOA (87 rows), cached publications (several newly cached:
PMID:15473967, 17682047, 20403325, 21529751, 27553190, 21394081, 25245017, 35493069).

Molecular function

• Binds dynein IC and LC8, not the motor domain PMID:17682047.
• Recruits LIS1 to dynein PMID:20403325.
• Patient truncations lose dynein binding PMID:21529751.
• RAB9 effector PMID:34793709.
• Dimers/tetramers, direct NDE1-NDEL1 interaction PMID:22843697.
• GOA has no dynein complex binding for NDE1 (or NDEL1), while LIS1, BICD2, NUMA1 carry GO:0070840 -> proposed NEW.

Localization

• Centrosome/mother centriole, spindle, kinetochores (CENP-F dependent) PMID:17600710.
• Nuclear envelope in prophase via NUP133-CENP-F PMID:21383080 -> NEW GO:0005635.

Processes

• Kinetochore dynein and metaphase alignment PMID:17600710.
• Apical INM in rat radial glia, NDE1-specific [PMID:27553190 "However, NDE1 inhibition alone causes a complete block of apical INM"; "RNAi against the NDE1 paralogue NDEL1 has no such effects."]; CDK1 sites T215/T243 PMID:39167527.
• Postmitotic neuronal migration: NDE1 and NDEL1 comparable PMID:27553190.
• Microcephaly/microlissencephaly [PMID:21529751, PMID:21529752]; mouse Nde1-null microcephaly with spindle orientation and centrosome duplication defects PMID:15473967.
• Cilium length restriction at mother centriole PMID:21394081 (mouse/zebrafish) - noted in description, not added as NEW.
• Nuclear pool / heterochromatin replication PMID:25245017 - noted as a question.

Decisions of note

• Kinesin complex IBA -> REMOVE (NDE1 is a dynein regulator, not a kinesin subunit).
• Protein binding rows -> REMOVE, except NDEL1 (22843697) -> MODIFY to protein heterodimerization activity.
• identical protein binding -> MODIFY to protein homodimerization activity.
• Chromosome localization IMP -> MODIFY to metaphase chromosome alignment.

Relevance to nucleokinesis module

• Module lumps NDE1/NDEL1 as one NudE unit. Data show asymmetry: NDE1 (not NDEL1) needed for apical INM in radial glia, both comparable in postmitotic migration; NDEL1 overexpression rescues INM.

Deep research incorporated (NDE1-deep-research-falcon.md, arrived after first draft)

• Pointed to Zhao et al. 2023 PMID:37940657 - cached and used to support NEW dynein complex binding / adaptor MF and core function.
• Confirms NDE1 (not NDEL1) requirement for apical INM; NDE1 enriched in progenitors, NDEL1 in postmitotic neurons (Tsai 2024, not cached).
• Cilium disassembly complex (Maskey 2015, Gabriel 2016) and proteasome association (Monda 2018) noted but not cached/annotated.