Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Combined Automated Annotation using Multiple IEA Methods
The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are required for [4Fe-4S] protein maturation.
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ISCA1 localizes to mitochondria
"ISCA1, ISCA2, and IBA57 are localized to mitochondria"
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ISCA1 depletion causes mitochondrial ultrastructural abnormalities
"Depleted cells contained massively swollen and enlarged mitochondria that were virtually devoid of cristae membranes"
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ISCA1 depletion reduces activity of [4Fe-4S] proteins (aconitase, complex I, LIAS)
"The activities of mitochondrial [4Fe-4S] proteins, including aconitase, respiratory complex I, and lipoic acid synthase, were diminished following depletion of the three proteins."
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[2Fe-2S] proteins (ferrochelatase) are unaffected by ISCA1 depletion
"In contrast, the mitochondrial [2Fe-2S] enzyme ferrochelatase and cellular heme content were unaffected."
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ISCA1 functions late in the ISC assembly pathway
"ISCA1, ISCA2, and IBA57 are specifically involved in the maturation of mitochondrial [4Fe-4S] proteins functioning late in the ISC assembly pathway."
Formation of [4Fe-4S] clusters in the mitochondrial iron-sulfur cluster assembly machinery.
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ISCA1/ISCA2 heterodimer receives [2Fe-2S] clusters from GLRX5
"two molecules of [2Fe-2S](2+) GRX5 donate their cluster to a heterodimeric ISCA1/ISCA2 complex."
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The heterodimer acts as an assembler converting two [2Fe-2S] to one [4Fe-4S]
"This complex acts as an "assembler" of [4Fe-4S] clusters; i.e., the two GRX5-donated [2Fe-2S](2+) clusters generate a [4Fe-4S](2+) cluster."
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[4Fe-4S] clusters are transferred to target apoproteins
"heterodimeric complex is the functional unit in mitochondria receiving [2Fe-2S] clusters from hGRX5 and assembling [4Fe-4S] clusters before their transfer to the final target apo proteins."
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ISCA1/ISCA2 is the functional unit for mitochondrial [4Fe-4S] assembly
"heterodimeric complex is the functional unit in mitochondria receiving [2Fe-2S] clusters from hGRX5 and assembling [4Fe-4S] clusters before their transfer to the final target apo proteins."
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
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BioPlex 3.0 interactome study confirms ISCA1-ISCA2 protein-protein interaction
"we have created two proteome-scale, cell-line-specific interaction networks"
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
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ISCA1 identified as mitochondrial protein in high-confidence mitochondrial proteome (MitoCoP)
"We classified >8,000 proteins in mitochondrial preparations of human cells and defined a mitochondrial high-confidence proteome of >1,100 proteins (MitoCoP)."
Multimodal cell maps as a foundation for structural and functional genomics.
Formation of 4Fe-4S cluster on ISCA1:ISCA2
Transfer of Fe-S clusters to SDHB
Deep research report on ISCA1