GO annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified annotations to orthologs by curator judgment
Annotation inferences using phylogenetic trees
GO annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
GO annotation based on curation of immunofluorescence data (HPA)
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
OpenScientist hypothesis investigation - CRMP1 cyclic-amide hydrolase activity
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OpenScientist judged the CRMP1 GO:0016812 IBA annotation to be an over-annotation and recommended removing it.
"No experimental evidence — direct assay, mutant phenotype, or otherwise — has ever demonstrated hydrolase activity for any CRMP family member."
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The report found no experimental hydrolase evidence for CRMP family members and traced the annotation to computational propagation from true dihydropyrimidinases.
"A systematic search of GO annotations for all five human CRMP paralogs (CRMP1-5/DPYSL1-5) found zero experimental evidence codes (IDA, IMP, IGI, IEP) supporting hydrolase activity for any CRMP family member."
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The report supports loss of cyclic-amide catalysis from missing metal-center residues and CRMP2 structural evidence. The CRMP5 negative assay is paralog evidence. Neither loss of this reaction nor a noncatalytic alternative role proves exclusion from all broader metabolic processes.
UniProt entry for CRMP1
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CRMP1 lacks the metal-cofactor-binding residues required for dihydropyrimidinase activity.
"Lacks most of the conserved residues that are essential for"
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CRMP1 belongs to the metallo-dependent hydrolase superfamily.
"Belongs to the metallo-dependent hydrolases superfamily."
Collapsin response mediator protein-1 and the invasion and metastasis of cancer cells.
A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease.
A human protein-protein interaction network: a resource for annotating the proteome.
From midbody protein-protein interaction network construction to novel regulators in cytokinesis.
A directed protein interaction network for investigating intracellular signal transduction.
Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Amino- and carboxyl-terminal domains of Filamin-A interact with CRMP1 to mediate Sema3A signalling.
A proteome-scale map of the human interactome network.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution.
Reactome pathway (CRMP/semaphorin signalling)
Reactome pathway (CRMP/semaphorin signalling)
Reactome pathway (CRMP/semaphorin signalling)
Collapsin response mediator protein 2: high-resolution crystal structure sheds light on small-molecule binding, post-translational modifications, and conformational flexibility.
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A human CRMP2 structure establishes loss of the ancestral dihydropyrimidinase active site; this is target evidence for DPYSL2 and comparative evidence for other CRMPs.
"Although CRMP-2, and other CRMPs, belong to the
dihydropyrimidinase family, they have lost the enzymatic active site."
Insights into the oligomerization of CRMPs: crystal structure of human collapsin response mediator protein 5.
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CRMP5 was directly tested for amidohydrolase activity and showed none; this is comparative, not a direct assay of the present target. CRMP1/2 oligomerization was also compared.
"CRMP-5 does not have any detectable amidohydrolase
activity."