GO reference used by source annotation pipeline
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Source annotation pipeline provenance for Ephx1 annotations including catalytic activity; ether hydrolase activity.
"GO_REF entry used only to trace the source annotation method; biological support was assessed against UniProt and cached literature where available."
GO reference used by source annotation pipeline
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Source annotation pipeline provenance for Ephx1 annotations including arachidonate metabolic process; cis-stilbene-oxide hydrolase activity; oxysterol binding.
"GO_REF entry used only to trace the source annotation method; biological support was assessed against UniProt and cached literature where available."
GO reference used by source annotation pipeline
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Source annotation pipeline provenance for Ephx1 annotations including arachidonate metabolic process; epoxide hydrolase activity; epoxide metabolic process.
"GO_REF entry used only to trace the source annotation method; biological support was assessed against UniProt and cached literature where available."
GO reference used by source annotation pipeline
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Source annotation pipeline provenance for Ephx1 annotations including endoplasmic reticulum membrane.
"GO_REF entry used only to trace the source annotation method; biological support was assessed against UniProt and cached literature where available."
GO reference used by source annotation pipeline
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Source annotation pipeline provenance for Ephx1 annotations including oxysterol binding.
"GO_REF entry used only to trace the source annotation method; biological support was assessed against UniProt and cached literature where available."
GO reference used by source annotation pipeline
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Source annotation pipeline provenance for Ephx1 annotations including epoxide hydrolase activity.
"GO_REF entry used only to trace the source annotation method; biological support was assessed against UniProt and cached literature where available."
GO reference used by source annotation pipeline
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Source annotation pipeline provenance for Ephx1 annotations including cis-stilbene-oxide hydrolase activity.
"GO_REF entry used only to trace the source annotation method; biological support was assessed against UniProt and cached literature where available."
GO reference used by source annotation pipeline
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Source annotation pipeline provenance for Ephx1 annotations including arachidonate metabolic process; cis-stilbene-oxide hydrolase activity; epoxide hydrolase activity; epoxide metabolic process; hydrocarbon catabolic process; and 1 other terms.
"GO_REF entry used only to trace the source annotation method; biological support was assessed against UniProt and cached literature where available."
Interaction between cytochrome P450 and other drug-metabolizing enzymes: evidence for an association of CYP1A1 with microsomal epoxide hydrolase and UDP-glucuronosyltransferase
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For Ephx1, this publication was reviewed as context for enzyme binding.
"Protein-protein interactions between cytochrome P450 (P450) and other drug-metabolizing enzymes were studied by affinity chromatography using CYP1A1-, glycine-, and bovine serum albumin (BSA)-conjugated Sepharose 4B columns."
EH3 (ABHD9): the first member of a new epoxide hydrolase family with high activity for fatty acid epoxides
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For Ephx1, this publication was reviewed as context for intracellular membrane-bounded organelle; membrane.
"Epoxide hydrolases are a small superfamily of enzymes important for the detoxification of chemically reactive xenobiotic epoxides and for the processing of endogenous epoxides that act as signaling molecules."
Glucocorticoid repression and basal regulation of the epoxide hydrolase promoter
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For Ephx1, this publication was reviewed as context for cellular response to glucocorticoid stimulus.
"Through a series of promoter deletions and gene transfer experiments we have examined the basal regulation and glucocorticoid-mediated repression of the rat epoxide hydrolase gene."
Quantitation of mRNAs specific for the mixed-function oxidase system in rat liver and extrahepatic tissues during development
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For Ephx1, this publication was reviewed as context for liver development.
"Evaluation of ontogenetic expression of the cytochrome P450PCN and cytochrome P450b gene families as well as the NADPH-cytochrome P450 oxidoreductase and epoxide hydrolase genes in Holtzmann rats showed that basal levels of mRNAs encoding these enzymes could be detected in most tissues."
Epoxide hydratase: sex specific expression and rate-limiting role in DMBA metabolism
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For Ephx1, this publication supports direct annotations to diol biosynthetic process.
"However, the sum of products that potentially derive from the common intermediate DMBA 3,4-oxide namely, DMBA 3,4-diol, 3- and 4-hydroxy DMBA, was comparable between the two sexes (18 versus 20 pmol/mg/min)."
Studies on the importance of microsomal epoxide hydrolase in the detoxification of arene oxides using the heterologous expression of the enzyme in mammalian cells
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For Ephx1, this publication supports direct annotations to epoxide hydrolase activity.
"In order to investigate the role of the microsomal epoxide hydrolase (mEH) in the detoxification of arene oxides in the presence of a high endogenous glutathione S-transferase (GST) activity-a situation found in several organs--we expressed the rat mEH cDNA in BHK21 Syrian hamster cells."
Expression of rat microsomal epoxide hydrolase in Escherichia coli. Identification of a histidyl residue essential for catalysis
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For Ephx1, this publication supports direct annotations to epoxide hydrolase activity.
"The cDNA containing the complete coding region for rat microsomal epoxide hydrolase (EC 3.3.2.3) was cloned into the expression/secretion vector pIN-III-OmpA3 and expressed in Escherichia coli strain TG1."
The membrane anchor of microsomal epoxide hydrolase from human, rat, and rabbit displays an unexpected membrane topology
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For Ephx1, this publication was reviewed as context for intracellular membrane-bounded organelle; membrane.
"Erratum in Biochem Biophys Res Commun."
Catalytic triad of microsomal epoxide hydrolase: replacement of Glu404 with Asp leads to a strongly increased turnover rate
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For Ephx1, this publication provides evidence/provenance for direct annotations to cis-stilbene-oxide hydrolase activity; epoxide hydrolase activity; epoxide metabolic process and contextual annotations to intracellular membrane-bounded organelle; membrane.
"Microsomal epoxide hydrolase (mEH) belongs to the superfamily of alpha/beta-hydrolase fold enzymes. A catalytic triad in the active centre of the enzyme hydrolyses the substrate molecules in a two-step reaction via the intermediate formation of an enzyme-substrate ester."
Falcon (Edison Scientific) deep research: Rat Ephx1 (UniProt P07687), microsomal epoxide hydrolase 1 (mEH; EC 3.3.2.9)
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Confirms gene identity as the microsomal epoxide hydrolase whose primary activity is hydrolysis of epoxides to vicinal diols, central to xenobiotic detoxification.
"Rat **Ephx1** encodes **microsomal epoxide hydrolase 1 (EPHX1/mEH)**, a membrane-anchored **α/β-hydrolase-fold** enzyme best known for catalyzing the **hydrolysis of epoxides to the corresponding vicinal diols**. This reaction is central to **xenobiotic detoxification** (and in some cases **bioactivation**) because many epoxides are reactive intermediates generated by cytochrome P450s."
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Distinguishes Ephx1 (microsomal mEH) from the soluble epoxide hydrolase EPHX2/sEH, confirming the correct target protein.
"It must be distinguished from **EPHX2**, the **soluble epoxide hydrolase (sEH)**, which is cytosolic/peroxisomal and has a different physiological emphasis in lipid-epoxide signaling."
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Describes the two-step covalent catalytic mechanism and the conserved Asp226-Glu404-His431 catalytic triad of mEH.
"Catalysis proceeds by a **two-step covalent mechanism** requiring a conserved catalytic triad **Asp226–Glu404–His431**, with Tyr residues contributing to epoxide activation/positioning."
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Localizes mEH primarily to the endoplasmic reticulum (microsomes) via an N-terminal membrane anchor.
"EPHX1 is primarily localized to the **endoplasmic reticulum (microsomes)** with a single **N-terminal membrane anchor**"
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Supports a role in arachidonate/fatty-acid epoxide metabolism, hydrolyzing EETs and EpOMEs to their corresponding diols.
"arachidonic-acid-derived **epoxyeicosatrienoic acids (EETs)** and linoleic-acid-derived **EpOMEs**, which are hydrolyzed to corresponding diols (**DHETs** and **DiHOMEs**)."
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Reports a non-canonical activity hydrolyzing the endocannabinoid 2-arachidonoylglycerol to arachidonic acid and glycerol.
"metabolism of the endocannabinoid **2-arachidonoylglycerol (2-AG)** to **arachidonic acid + glycerol**"
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Reports epoxysteroids (e.g. androstene oxide) among endogenous substrates, consistent with oxysterol/steroid-epoxide binding context.
"**epoxysteroids** such as androstene oxide/estroxide"
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Notes additional detection at the hepatocyte sinusoidal/plasma membrane linked to bile acid transport, supporting non-core membrane localization beyond the ER.
"EPHX1 has also been detected at the **hepatocyte sinusoidal/plasma membrane**, where it has been linked to **sodium-dependent bile acid transport** phenomena in hepatocytes"