Affinage mechanistic annotation for ADAMTSL5 (human) Affinage Affinage (Claude Sonnet reading pass + Opus synthesis pass) 7 citations

Affinage mechanistic annotation for ADAMTSL5 (human)

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Current model (mechanistic narrative)

ADAMTSL5 is a secreted, N-glycosylated extracellular matrix glycoprotein that associates with fibrillin microfibrils and also functions as a disease-relevant signaling modulator and autoantigen [PMID:23010571, PMID:33197513, PMID:26621454]. As a matrix component, it binds both fibrillin-1 and fibrillin-2, co-localizes with fibrillin microfibrils in fibroblast cultures, and binds heparin through its C-terminal netrin-like (NTR) module, which can be proteolytically released PMID:23010571. In hepatocellular carcinoma, ADAMTSL5 sustains oncogenic receptor tyrosine kinase signaling: its depletion lowers expression and/or phosphorylation of MET, EGFR, PDGFRβ, IGF1Rβ, and FGFR4 while raising AXL, and its overexpression confers tumorigenicity to MET-sensitized hepatocytes, with its own expression linked to gene-body CpG island hypermethylation PMID:33197513. In psoriasis, ADAMTSL5 is an HLA-C06:02–presented melanocyte autoantigen whose VRSRRCLRL peptide is recognized by an autoreactive Vα3S1/Vβ13S1 CD8+ TCR, driving IL-17A responses PMID:26621454; the structural basis of this recognition is an extensive complementary electrostatic interface between negatively charged TCR residues and exposed arginines of the self-peptide and the HLA-C06:02 α1 helix PMID:37330172. This TCR is polyspecific, also responding to environmental peptides from wheat, microbiota, and pathogens, providing a route by which environmental antigens may trigger the ADAMTSL5-directed autoimmune response PMID:38524140.

Affinage mechanism profile (its own GO/Reactome grounding)

Recorded for reference. The AIGR evaluation found this grounding is coarse (collapses to general parents) and can contradict the narrative — do not import these GO ids directly; re-ground from the narrative + PMIDs.

Dated findings (citation-anchored)

Year Confidence Finding PMIDs Journal
2015 High ADAMTSL5 was identified as an HLA-C*06:02-presented melanocytic autoantigen recognized by a Vα3S1/Vβ13S1 T cell receptor (TCR) reconstituted from an epidermal CD8+ T cell clone of a psoriasis patient. Melanocytes are the skin-specific target cells expressing ADAMTSL5, and ADAMTSL5 stimulation induced IL-17A in CD8+ T cells from psoriasis patients only. PMID:26621454 The Journal of experimental medicine
2012 High ADAMTSL5 is a secreted, N-glycosylated ~60 kDa glycoprotein that binds both fibrillin-1 and fibrillin-2 (the first ADAMTS family member shown to bind both), co-localizes with fibrillin microfibrils in the extracellular matrix of cultured fibroblasts, and binds heparin via its C-terminal netrin-like (NTR) module. Proteolytic release of the NTR module was also observed. Alternative splicing at the 5' end generates two transcripts encoding different signal peptides but the same mature protein, with differing translational efficiency. PMID:23010571 Matrix biology : journal of the International Society for Matrix Biology
2023 High The crystal structure of the psoriatic Vα3S1/Vβ13S1 TCR in complex with HLA-C06:02 presenting the ADAMTSL5 peptide (VRSRRCLRL) was determined. TCR docking involves an extensive complementary charge network between negatively charged TCR residues and exposed arginine residues from the ADAMTSL5 self-peptide and the HLA-C06:02 α1 helix. Mutagenesis and activation assays confirmed these electrostatic interactions are functionally critical. PMID:37330172 The Journal of biological chemistry
2020 High ADAMTSL5 maintains the function of key oncogenic signaling pathways in hepatocellular carcinoma (HCC). ADAMTSL5 depletion reduced expression and/or phosphorylation of receptor tyrosine kinases MET, EGFR, PDGFRβ, IGF1Rβ, and FGFR4, and increased AXL expression. Conversely, ADAMTSL5 overexpression conferred tumorigenicity to pre-tumoural hepatocytes sensitized by modest MET receptor expression. ADAMTSL5 expression correlates with gene body CpG island hypermethylation at its locus. PMID:33197513 Journal of hepatology
2024 Medium Multiple environmental peptides (from wheat, Saccharomyces cerevisiae, microbiota, tobacco, and pathogens) activate the same psoriatic Vα3S1/Vβ13S1 TCR that recognizes ADAMTSL5, as demonstrated by lymphocyte stimulation experiments. HLA-C*06:02 tetramers loaded with ADAMTSL5 or wheat peptides showed the same CD8+ T cell population can recognize both, establishing TCR polyspecificity as a mechanism by which environmental antigens may trigger the ADAMTSL5-directed autoimmune response. PMID:38524140 Frontiers in immunology
2016 Medium ADAMTSL5 protein is expressed not only in epidermal melanocytes but also in keratinocytes throughout the epidermis and in some dermal blood vessels and perivascular dermal cells in psoriatic skin, as shown by immunohistochemistry with three different antibodies. PMID:27857980 Journal of pigmentary disorders
2017 Medium ADAMTSL5 and LL37 protein levels are significantly increased in lesional psoriatic skin and are co-expressed by dendritic cells, macrophages, and some T cells in the dermis. ADAMTSL5 expression is significantly downregulated following treatment with IL-17 or TNF-α blockade, indicating that psoriasis-related cytokines feed-forward induction of ADAMTSL5. PMID:28482118 Experimental dermatology

Citations