Gene Ontology annotation through association of InterPro records with GO terms.
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara.
Combined Automated Annotation using Multiple IEA Methods.
Defining the membrane proteome of NK cells.
Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
A genetic screen in Drosophila reveals an unexpected role for the KIP1 ubiquitination-promoting complex in male fertility.
The human archain gene, ARCN1, has highly conserved homologs in rice and Drosophila.
Active ARF recruits coatomer
ARFGAP, cargo, v-SNAREs and p24 proteins bind nascent COPI complex
ARFGAPs stimulate ARF GTPase activity
ERGIC-to-Golgi vesicles bind dynein:dynactin
Vesicle is tethered through binding GOLGA2:GORASP1, GOLGB1 and the COG complex
cis-Golgi t-SNAREs bind YKT6 on tethered vesicle
Active ARF recruits coatomer to the Golgi
ARFGAP, cargo, vSNARES and p24 proteins bind COPI vesicles at Golgi
ARFGAPs stimulate ARF GTPase activity at the Golgi membrane
Retrograde vesicle is tethered at the ER by the NRZ complex and t-SNAREs
Retrograde COPI vesicles bind kinesin and microtubules
COPI vesicle uncoating at the ER
Deep research on ARCN1 function
Deep research on ARCN1 function (Cyberian)
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Delta-COP contains an N-terminal longin domain essential for COPI function
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A critical amphipathic helix C-terminal to the longin domain is specifically required for HDEL-bearing ER-luminal protein retrieval
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The C-terminal mu-homology domain (MHD) recognizes di-tryptophan motifs in cargo and tethering factors
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Delta-COP together with beta-COP recognizes arginine (R)-based ER localization signals for quality control
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Heterozygous ARCN1 mutations cause short stature-micrognathia syndrome (OMIM 617164)
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ARCN1 deficiency causes ER stress and impaired collagen secretion
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The nur17 mouse model demonstrates tissue-specific requirements in Purkinje neurons and melanocytes
Architecture of coatomer - molecular characterization of delta-COP and protein interactions within the complex
Delta- and zeta-COP, two coatomer subunits homologous to clathrin-associated proteins, are involved in ER retrieval
ARCN1 Mutations Cause a Recognizable Craniofacial Syndrome Due to COPI-Mediated Transport Defects
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Heterozygous loss-of-function mutations in ARCN1 cause short stature-micrognathia syndrome
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ARCN1 deficiency triggers ER stress response with upregulation of ATF4, CHOP, and BiP
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Reduced ARCN1 causes intracellular accumulation of type I collagen with reduced secretion
Mutation in Archain 1, a Subunit of COPI Coatomer Complex, Causes Diluted Coat Color and Purkinje Cell Degeneration
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The nur17 mouse I422T mutation causes coat color dilution and progressive cerebellar ataxia
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Purkinje cell degeneration with abnormal protein accumulation and ER stress markers
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Impaired Tyrp1 glycosylation in melanocytes demonstrates altered ER-Golgi trafficking
δ-COP contains a helix C-terminal to its longin domain key to COPI dynamics and function.
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The N-terminal longin domain is essential for COPI function in early secretory pathway
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An amphipathic helix C-terminal to longin domain is required for HDEL-bearing protein retrieval
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Bovine delta-COP can functionally substitute for yeast RET2 despite only 34% sequence similarity
Novel cargo-binding site in the beta and delta subunits of coatomer
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Beta-COP and delta-COP together recognize arginine (R)-based ER localization signals
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Mutations in both subunits abolish R-based signal recognition while leaving dilysine recognition intact
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R-based signal binding site occupies same structural position as YXXF recognition in clathrin adaptors
9Å structure of the COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments.
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Cryo-EM at 9 angstrom resolution with 2.57 angstrom crystal structure of beta-delta-COP complex
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Arf1 occupies two distinct environments - gamma-Arf1 accessible to ArfGAP and beta-Arf1 contacting delta-COP
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Delta-COP helices directly contact Arf1 switch regions stabilizing GTP-bound state
COPI Budding within the Golgi Stack
Dsl1p, an essential component of the Golgi-endoplasmic reticulum retrieval system in yeast, uses the same sequence motif to interact with different subunits of the COPI vesicle coat.
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Dsl1 central acidic domain binds directly to delta-COP
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Dsl1 uses same domain to interact with both delta-COP and alpha-COP
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Dsl1 binding may promote coat disassembly while tethering the vesicle
A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1.
Molecular Basis for Recognition of Dilysine Trafficking Motifs by COPI
Biochemical heterogeneity and phosphorylation of coatomer subunits
Directing Traffic - Regulation of COPI Transport by Post-translational Modifications
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PKA-mediated phosphorylation of alpha-, delta-, epsilon-, and zeta-COP
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Multiple kinases regulate COPI function including Src, PKC, AMPK, casein kinases, and LRRK2