Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Towards a proteome-scale map of the human protein-protein interaction network.
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Large-scale Y2H interactome mapping study
"Here we describe an initial version of a proteome-scale map of human binary protein-protein interactions"
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Does not provide specific functional information about ADAMTSL4
Cathepsin B and its interacting proteins, bikunin and TSRC1, correlate with TNF-induced apoptosis of ovarian cancer cells OV-90.
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Identified ADAMTSL4 (TSRC1) as cathepsin B binding protein via Y2H
"Two cathepsin B binding proteins, bikunin and TSRC1, were identified by yeast two-hybrid method and the interactions were confirmed in vitro and in vivo"
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Overexpression of TSRC1 had effect on TNF-induced apoptosis in cancer cells
"TSRC1 overexpression had an opposite effect on apoptosis"
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Study context is cancer biology, not ADAMTSL4's normal physiological function
An empirical framework for binary interactome mapping.
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Methodological study on interactome mapping quality assessment
"Here we describe a framework that uses an empirically-based approach to rigorously dissect quality parameters"
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No specific information about ADAMTSL4 function
ADAMTSL4, a secreted glycoprotein widely distributed in the eye, binds fibrillin-1 microfibrils and accelerates microfibril biogenesis.
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Key study establishing ADAMTSL4's core function
"ADAMTSL4 is a secreted glycoprotein that is widely distributed in the human eye"
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Demonstrates binding to fibrillin-1 microfibrils
"ADAMTSL4 colocalized with fibrillin-1 microfibrils in the ECM of these cells"
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Shows ADAMTSL4 accelerates microfibril biogenesis
"Enhanced fibrillin-1 deposition in the presence of ADAMTSL4"
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Establishes ECM localization in the eye
Protein interactions of the transcription factor Hoxa1.
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Y2H screen identified ADAMTSL4 among 59 Hoxa1 interactors
"To investigate the mode of action of mammalian Hoxa1, we characterized its interactome by a systematic yeast two-hybrid screening against ~12,200 ORF-derived polypeptides"
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Biological relevance of this interaction to ADAMTSL4 function is unclear
Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver.
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Proteomic study detecting ADAMTSL4 in ECM-enriched fractions
"We have used enrichment of extracellular matrix (ECM) from human patient samples and proteomics to define the ECM composition of primary colon carcinomas and their metastases to liver"
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Confirms ECM localization
Comprehensive proteomic characterization of stem cell-derived extracellular matrices.
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Mass spectrometry detection of ADAMTSL4 in ECM preparations
"Here, we characterized and compared the protein composition of ECM produced in vitro by bone marrow-derived MSC, adipose-derived MSC and neonatal fibroblasts from different donors, employing quantitative proteomic methods"
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Confirms ECM localization
Interactions between lysyl oxidases and ADAMTS proteins suggest a novel crosstalk between two extracellular matrix families.
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Y2H screen identified ADAMTSL4 as LOX binding protein
"A yeast two-hybrid screen to identify lysyl oxidase (LOX) binding proteins identified ADAMTSL4 as a potential interactor"
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Suggests functional crosstalk between ECM protein families
B3GALTL transfers glucose to O-fucosyl-proteins
POFUT2 transfers fucose to TSR domain-containing proteins
Defective B3GALTL does not transfer glucose to O-fucosyl-proteins
A homozygous mutation in ADAMTSL4 causes autosomal-recessive isolated ectopia lentis.
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Establishes ADAMTSL4 as disease gene for ectopia lentis
"mutations in ADAMTSL4 are responsible for autosomal-recessive simple ectopia lentis"
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Demonstrates zonular fiber function
Deep research summary for ADAMTSL4