GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000003

Gene Ontology annotation based on Enzyme Commission mapping

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000118

TreeGrafter-generated GO annotations

file:NICAT/NaUGT1_candidate_UGT85A2_0/NaUGT1_candidate_UGT85A2_0-uniprot.txt

UniProt entry A0A2H4GSI3 for Nicotiana attenuata UGT85A2_0

UGT85A2_0 is a UDP-glycosyltransferase family enzyme

"CC -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family."
The public UniProt entry carries family-derived glucosyltransferase annotations

"DE RecName: Full=7-deoxyloganetic acid glucosyltransferase"

file:NICAT/NaUGT1_candidate_UGT85A2_0/NaUGT1_candidate_UGT85A2_0-notes.md

NaUGT1 UGT85A2_0 candidate notes

UGT1 is the NaGT step in the reconstituted nicotine synthase cascade

"The glucosylation preprint makes UGT1 a core late-pathway enzyme by reconstituting the four-enzyme nicotine synthase cascade with UGT1, A622, BBLa, and beta-GD1, and it explicitly assigns UGT1 = NaGT, the nicotinic acid N-glucosyltransferase step."
UGT85A2_0 is the best current sequence-backed NICAT ortholog to tobacco UGT1

"The 2026-04-05 mapping dive assigns NaUGT1 to UGT85A2_0 / A0A2H4GSI3 as the best current sequence-backed NICAT ortholog to tobacco UGT1."

file:NICAT/NaUGT1_candidate_UGT85A2_0/NaUGT1_candidate_UGT85A2_0-deep-research-falcon.md

Deep research report on NaUGT1/UGT85A2_0 (Falcon/Edison Scientific Literature)

The earlier report lacked a primary sequence crosswalk for the candidate and therefore treated substrate assignment as unresolved. The independent primary-sequence analysis below now supplies that crosswalk at the gene level.

file:NICAT/NaUGT1_candidate_UGT85A2_0/NaUGT1_candidate_UGT85A2_0-hypotheses/function-hypothesis-go-0080043/openscientist.md

Blinded OpenScientist function-assignment report (TreeGrafter audit)

file:NICAT/NaUGT1_candidate_UGT85A2_0/NaUGT1_candidate_UGT85A2_0-hypotheses/function-hypothesis-go-0080043/falcon.md

Existing falcon function-hypothesis report

PMID:42151135

Nicotine biosynthesis is completed by cryptic activating glucosylation.

The purified tobacco UGT1 enzyme N-glucosylates nicotinic acid and participates in the reconstituted nicotine biosynthesis cascade. Supplementary sequences explicitly identify the assayed homolog.

"producing nicotinic acid N-glucoside ( 10) from nicotinic acid ( 2) and UDP-glucose"

PMID:41928514

Complete biosynthesis of nicotine.

The independent N. attenuata study biochemically assays NaUGT1 and disrupts its locus; primary supplemental primers link those assays to g26396, with an N-terminal construct extension documented in the identity analysis.

"Abrogating any of these components depletes nicotine accumulations."

PMID:31611893

Qualitative and Quantitative NAD(+) Metabolomics Lead to Discovery of Multiple Functional Nicotinate N-Glycosyltransferase in Arabidopsis.

Arabidopsis UGT76C4/UGT76C5 are experimentally characterized nicotinate N-glycosyltransferases, contradicting the report claim that no plant examples were known.

"Previously, we had identified UGT76C4 and UGT76C5 as NA N-glucosyltransferase"

file:NICAT/NaUGT1_candidate_UGT85A2_0/NaUGT1_candidate_UGT85A2_0-bioinformatics/RESULTS.md

Reproducible NaUGT1 primary-sequence and ontology reconciliation

Explicit primary accession mapping and an independently recomputed global alignment support the close tobacco homolog; the gene-level Cell evidence resolves the candidate identity.

"The assayed tobacco UGT1 maps explicitly to A0A1S3YWH6 and is a nicotinate N-glucosyltransferase."

file:NICAT/NaUGT1_candidate_UGT85A2_0/NaUGT1_candidate_UGT85A2_0-bioinformatics/identity-cell.md

Cell 2026 NaUGT1 identity check against primary primers and GenBank sequences

Published cloning and perturbation primers map to the g26396 locus represented by A0A2H4GSI3; a primer-compatible extended ORF contains its entire 485-aa sequence unchanged.

"Figure 3D assays purified recombinant NaUGT1 expressed in E. coli and detects nicotinic acid N-glucoside from nicotinic acid."

file:NICAT/NaUGT1_candidate_UGT85A2_0/NaUGT1_candidate_UGT85A2_0-hypotheses/naugt1-candidate-identity-and-nicotinate-glucosylation/openscientist.md

OpenScientist adjudication of NaUGT1 identity and nicotinate glucosylation

The report claims nicotinate N-glucosylation is outside nicotine biosynthesis.

"nicotinic acid *N*-glucoside is a separate conjugation/storage branch (one of three fates of nicotinic acid), not a nicotine-biosynthetic step."