Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Peroxisomal alanine : glyoxylate aminotransferase (AGT1) is a photorespiratory enzyme with multiple substrates in Arabidopsis thaliana.
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Recombinant AGT1 catalyses Ala:glyoxylate, Ser:glyoxylate and Ser:pyruvate transamination, prefers Ser:glyoxylate, is peroxisomal, and carries the sat mutation.
"We conclude that Arabidopsis AGT1 is a peroxisomal photorespiratory enzyme that catalyzes transamination reactions with multiple substrates."
Large-scale analysis of in vivo phosphorylated membrane proteins by immobilized metal ion affinity chromatography and mass spectrometry.
Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity.
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sat mutants completely lack SGAT activity, are inviable under photorespiratory conditions and accumulate serine and glycine.
"Serine and glycine accumulated as end products of photosynthesis in the mutants"
Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms.
Properties of serine:glyoxylate aminotransferase purified from Arabidopsis thaliana leaves.
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics; an oxidative stress case study.
Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis.
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
Photorespiration.
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SGAT is a single gene whose deletion is lethal in normal air.
"In Arabidopsis, SGAT is encoded by a single gene (At2g13360) and its deletion results in plants that are unviable under normal atmospheric conditions"
Characterization of Arabidopsis serine:glyoxylate aminotransferase, AGT1, as an asparagine aminotransferase.
RabGAP22 is required for defense to the vascular pathogen Verticillium longisporum and contributes to stomata immunity.
A proteomic strategy for global analysis of plant protein complexes.
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Crystal Structure Of Photorespiratory Alanine:Glyoxylate Aminotransferase 1 (AGT1) From Arabidopsis thaliana.
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AGT1 is a PLP-binding homodimer; serine-binding residues from the partner subunit explain its serine preference; the sat P251L lesion lies at the dimer interface.
"The structure also rationalizes the phenotype of the sat mutant, Pro251 to Leu, which likely affects the dimer interface near the catalytic site."
Discovering the RNA-Binding Proteome of Plant Leaves with an Improved RNA Interactome Capture Method.