PANTHER family review PTHR19918: IBA propagation assessment for slp1
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
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Phylogenetic (PANTHER) inference of APC/C component, APC/C binding, ubiquitin ligase activator activity, and positive regulation of APC/C-dependent catabolic process, all consistent with experimental evidence in S. pombe.
Electronic Gene Ontology annotations created by ARBA machine learning models
Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p.
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Mes1 interacts with Slp1 and blocks degradation of cyclin Cdc13 to control meiotic progression; basis for an Slp1-Mes1 protein-interaction annotation.
"Fission yeast Mes1p ensures the onset of meiosis II by blocking degradation of cyclin Cdc13p"
Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles.
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The Mis6 kinetochore subcomplex interacts with Mad2 when the checkpoint is active, establishing the kinetochore as the SAC signalling platform that controls Slp1.
"The Mis6-complex physically interacts with Mad2 under the condition that the Mad2-dependent checkpoint is activated"
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
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Proteome-scale GFP localization study; Slp1 localizes to nucleus and mitotic structures (spindle, spindle pole body, cell division site).
"we determined the localization of 4,431 proteins"
A mutual inhibition between APC/C and its substrate Mes1 required for meiotic progression in fission yeast.
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Mes1 binds the WD40 domain of Fizzy-family APC/C activators (including Slp1) and is both an inhibitor and a substrate, forming a ternary APC/C-Cdc20-Mes1 complex.
"Mes1 directly binds the WD40 domain of the Fizzy family of APC/C activators"
The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).
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Slp1/Cdc20 is the APC/C activator early in mitosis and the key SAC target; it is incorporated into the S. pombe MCC (Mad3-Mad2-Slp1) and cofractionates with the APC/C.
"abolishes incorporation of Mad3 into the mitotic checkpoint complex (Mad3-Mad2-Slp1 in S. pombe, where Slp1 is the Cdc20 homolog"
Mes1 controls the meiosis I to meiosis II transition by distinctly regulating the anaphase-promoting complex/cyclosome coactivators Fzr1/Mfr1 and Slp1 in fission yeast.
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Slp1/Cdc20 is the only coactivator essential for meiosis I progression; Mes1 acts as a competitive substrate for Slp1, distinct from its pseudosubstrate inhibition of Fzr1/Mfr1.
"find that only Slp1/Cdc20 is essential for meiosis I progression"
Structure of the mitotic checkpoint complex.
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The S. pombe MCC crystal structure (Mad2-Mad3-Cdc20/Slp1) reveals that the MCC inhibits the APC/C by obstructing Cdc20 degron-recognition sites and displacing Cdc20.
"The MCC inhibits the APC/C by obstructing degron recognition sites on Cdc20 (the substrate recruitment subunit of the APC/C) and displacing Cdc20 to disrupt formation of a bipartite D-box receptor with the APC/C subunit Apc10"
Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests.
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Cdc20Slp1-APC/C ubiquitinates securin (Cut2) in vitro; Mps1/Mph1 phosphorylation of Mad3 potentiates inhibition of Cdc20Slp1-APC/C.
"a corresponding decrease in Cdc20 Slp1-APC/C dependent ubiquitination of the radio-labelled securinCut2 substrate was observed"
Identification of a Sgo2-Dependent but Mad2-Independent Pathway Controlling Anaphase Onset in Fission Yeast.
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A Mad2-independent Mad3-Slp1(Cdc20) complex also inhibits the APC/C, with Mad3 binding Slp1 in a KEN20-dependent manner.
"Mad3 interacts with Slp1 in a cell cycle- and KEN20-dependent manner"
Fission yeast Slp1: an effector of the Mad2-dependent spindle checkpoint.
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Slp1 is a WD-repeat protein essential for anaphase onset that forms a complex with Mad2; disrupting the Slp1-Mad2 interaction abolishes the spindle checkpoint.
"Mad2 formed a complex with Slp1, a WD (tryptophan-aspartic acid)-repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional"