MAP1LC3B notes (human, Q9GZQ8)

Deep research: not run (falcon times out after 600 s in this environment and perplexity-lite is not
installed). Review based on cached publications and the UniProt record.

Key findings

• Cleaved at Gly120 by ATG4B and conjugated to PE by ATG7/ATG3 PMID:15355958. This corrects PMID:12740394, which reported no C-terminal cleavage.
• LC3 subfamily: phagophore elongation; GABARAPs: later maturation PMID:20418806.
• Atg8 hexa-KO: autophagosomes still form (smaller, slower), fusion fails; GABARAPs dominate fusion and PINK1/Parkin mitophagy PMID:27864321.
• LIR receptor docking: PHB2 PMID:28017329, AMBRA1 PMID:25215947; ceramide binding in lethal mitophagy PMID:22922758; JMY actin nucleation PMID:30420355.

Decisions

• 149 protein binding IPI rows: REMOVE (uninformative); there is no GO MF term for Atg8 LIR docking (raised as a suggested question).
• Core MF: phosphatidylethanolamine binding (repo convention for Atg8 lipidation, as in worm lgg-1).
• Mitochondrial locations, ceramide binding, ubiquitin ligase binding, starvation-response terms, cytoskeleton/axoneme/microtubule binding: non-core.