KGD4 (YFR049W / YMR31) notes

UniProt P19955; module tca_cycle (Kgd4/MRPS36 adaptor annoton, GO:0030674).

Evidence journal

• Kgd4 is a novel subunit of mitochondrial alpha-KGDH, previously assigned to the mitoribosome PMID:25165143.
• Adaptor mechanism: binds E1-E2 core and E3 PMID:25165143; direct Lpd1 binding PMID:25165143; C-terminal domain contacts the core PMID:25165143.
• Activity: PMID:25165143.
• Ribosome assignment is contamination PMID:25165143; UniProt CAUTION agrees [UniProt:P19955].
• Original ribosomal-protein claim: PMID:2693936.
• Isoforms Kgd4S/Kgd4L via alternative UUG initiation [UniProt:P19955].

Curation decisions

• REMOVE structural constituent of ribosome (GO:0003735) and mitochondrial ribosome (GO:0005761): contradicted by full-text evidence.
• Protein binding: MODIFY low-throughput rows to GO:0030674; REMOVE HTP rows.
• YeastCyc: KGD4 is not attached to the 2-oxoglutarate dehydrogenase reaction in the cached YeastPathways export, yet SGD's RCA rows (TCA-EUK-PWY) do include KGD4 with correct mitochondrial compartment.