Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
A proteome-scale map of the human interactome network.
Cloning and sequence analysis of human pituitary cDNA encoding the novel polypeptide 7B2.
A reference map of the human binary protein interactome.
7B2 is a neuroendocrine chaperone that transiently interacts with prohormone convertase PC2 in the secretory pathway.
Mechanism of the facilitation of PC2 maturation by 7B2 involvement in ProPC2 transport and activation but not folding.
Neuroendocrine secretory protein 7B2 structure, expression and functions.
Secreted Chaperones in Neurodegeneration
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7B2 is a neuroendocrine secreted chaperone protein packaged with neuropeptides and released during synaptic activity, with client binding influenced by its intrinsically disordered region and oligomerization behavior.
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Full-length 7B2 (~27 kDa) is cleaved by the Golgi-resident convertase furin to yield a ~21 kDa product; both forms contain a central intrinsically disordered region (IDR).
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CSF levels of 7B2 are approximately 3 ng/mL (0.14 nM) and decline with age, and SCG5 has been recurrently identified in AD vs control CSF proteomics studies.
Revisiting Proinsulin Processing -- Evidence That Human Beta-Cells Process Proinsulin With Prohormone Convertase (PC) 1/3 but Not PC2
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In human islets, PC2 immunoreactivity is primarily in alpha-cells and nearly undetectable in beta-cells, contrasting with mouse islets where both cell types express PC2.
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PC2 inhibition nearly blocks glucagon production but does not significantly change proinsulin processing in pulse-chase experiments on human islets.
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7B2 immunoreactivity is detectable in both alpha- and beta-cells in human islets, but the intensity is lower in beta-cells than alpha-cells.
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Prior reports indicate that 7B2 suppresses aggregation and cytotoxicity for amyloidogenic peptides including beta-amyloid, synuclein, and IAPP.
Proteomics of the astrocyte secretome reveals changes in their response to soluble oligomeric Aß
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SCG5 is among proteins with reported chaperone activity whose secretion is altered in astrocytes exposed to Abeta oligomer-containing medium.
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SCG5 has been identified in 4 of 10 AD vs control CSF proteomics studies reviewed, and chromogranins/secretogranins are proposed AD biomarkers with decreased CSF levels.
Cystic fibrosis-related diabetes is associated with reduced islet protein expression of GLP-1 receptor and perturbation of cell-specific transcriptional programs