Deep research: not run (falcon times out in this environment; perplexity-lite unavailable). Review based on cached publications and UniProt.
Drp1 performs GTP-driven membrane division rather than merely regulating another fission enzyme. The integrated motor core covers mitochondrial membrane fission and peroxisome fission, with a soluble cytosolic pool and peripheral organelle-membrane recruitment. Direct human reconstitution shows tube severing; the more specific claim of a resolved twisting geometry is not required. The historical 2013 construct sequences map to O00429-4 and O00429-3 as documented in the source guide; the 2018 construct name has not been equated to a modern accession. No equal activity or localization of all nine natural products is inferred. PMID:23530241(https://pubmed.ncbi.nlm.nih.gov/23530241/), PMID:23977156(https://pubmed.ncbi.nlm.nih.gov/23977156/), PMID:30531964(https://pubmed.ncbi.nlm.nih.gov/30531964/), PMID:12499366(https://pubmed.ncbi.nlm.nih.gov/12499366/), PMID:12618434(https://pubmed.ncbi.nlm.nih.gov/12618434/).
The inherited journal above records earlier decisions. This augmentation supersedes its generic-binding removals and cross-branch regulation replacements: positive associations are retained under the scoped project curation instructions, with specific partner-class terms only where actual evidence supports them. Rab32 binding establishes an effector/recruitment relationship, not a Rab GTPase-activator activity. Regulation of fission remains distinct from direct membrane fission. PMID:25767741(https://pubmed.ncbi.nlm.nih.gov/25767741/), PMID:19279012(https://pubmed.ncbi.nlm.nih.gov/19279012/), PMID:20436456(https://pubmed.ncbi.nlm.nih.gov/20436456/).
Localization is nonexclusive and source specific. The endogenous COS-7 ER-negative staining and human Drp1(T59A) transgene ER-morphology observations in COS-7 remain NOT assertions, with a documented context exception, alongside positive rat or condition-dependent ER/Golgi evidence. Human endogenous Jurkat microtubule association is distinct from rat splice constructs expressed in HeLa. Rat hippocampal immunogold and vesicle preparations support the neuronal coated-pit/vesicle pool without constituting a direct human neuronal assay. Mitochondrion-derived-vesicle presence describes cargo, not vesicle-production activity. PMID:11514614(https://pubmed.ncbi.nlm.nih.gov/11514614/), PMID:9472031(https://pubmed.ncbi.nlm.nih.gov/9472031/), PMID:20688057(https://pubmed.ncbi.nlm.nih.gov/20688057/), PMID:23798729(https://pubmed.ncbi.nlm.nih.gov/23798729/), PMID:23792689(https://pubmed.ncbi.nlm.nih.gov/23792689/), PMID:26618722(https://pubmed.ncbi.nlm.nih.gov/26618722/).
The three imported PMID37971521 rows remain unresolved at source level because the relevant supplemental target evidence was not recovered. The separately verified 2024 paper directly tests Drp1-dependent fragmentation and mitochondrial recruitment in human U2OS cells, with mouse Python experiments reported separately. This is independent corroboration, not a verified citation replacement. PMID:37971521(https://pubmed.ncbi.nlm.nih.gov/37971521/), PMID:38587461(https://pubmed.ncbi.nlm.nih.gov/38587461/).
PAINT judgments were checked against the actual family tree, target MSA sequence and literal ancestral IBD records. Target self-evidence and short donor lists are not defects. The mouse brush-border donor is real, but the supplemental Dnm1l target record remains unresolved. MitoCoP O00429 entries are literature-pool 4 selections, not new experimental target-localization validation. The mouse Python ATP/contraction findings are tissue-specific consequences of a C452F mutation, not ATP synthesis or contractile catalysis by Drp1. PMID:22114352(https://pubmed.ncbi.nlm.nih.gov/22114352/), PMID:34800366(https://pubmed.ncbi.nlm.nih.gov/34800366/), PMID:20585624(https://pubmed.ncbi.nlm.nih.gov/20585624/).
All 153 imported annotation source dictionaries, all 67 inherited reference identities and the prior notes prefix are preserved. No NEW process annotation is proposed. Selected existing quotation anchors are retained once where informative; the only newly reproduced publication excerpt is the eight-word PMID30531964 motor-core anchor. Cached full-text flags describe the selected immutable cache, while separate original-access scope is stated in the reference assessments and source guide. Genuine Falcon authentication and fallback quota failures are preserved in the research receipts; no provider report was fabricated.
The linked DNM1L source-evidence guide now identifies each annotation by its preserved term, evidence, original reference, qualifier and relevant partner/negation/product fields. These keys replace positional indices so the source associations survive list reordering. The guide is navigation and access provenance, not an independent experiment; its stable keys are checked against the review separately from the GeneReview schema.
The genuine PMID:21217774 publisher supplement, Supplementary Table 1, page 1, fourth data row, explicitly reports DNM1L clone BC024590. Its in-vitro result involves the ERalpha ligand-binding domain and depends on estradiol, while the mammalian two-hybrid result is estradiol independent. Both columns were independently checked in the rendered table as well as extracted text. The positive pair supports the existing nuclear-receptor-binding refinement beyond a discovery-screen hit. The main article's detailed purified-protein protocol and co-activation/migration experiments concern RAC3; they are not assigned to DNM1L. Native physiological interaction, transcriptional co-activation and current splice-product identity are not established by this target row. The guide contains the exact categorical record, original URL, PDF checksum and extraction command. Its supporting relevance is MEDIUM.
The cytosol IDA from GO_REF:0000052 now directly cites the Human Protein Atlas DNM1L record. The official XML identifies ENSG00000087470/O00429 and gives supported cytosol calls for two antibodies in A-431, U-251MG and U2OS. Additional vesicle calls are recorded without proposing a new localization annotation. This is a source-record assessment, not an independent microscopy-pixel interpretation.
PMID:21525035 is a genuine current human DNM2 donor citation: the saved QuickGO record joins P50570, microtubule GO:0005874, IDA and UniProt assignment dated 2012-06-01. Its abstract foregrounds PEX14, and the DNM2-specific full experiment remains unread. The literal donor record establishes provenance, not a reason to transfer the PEX14 mechanism to DNM1L or dismiss the donor because of the title.
All 153 source dictionaries and annotation actions, nine product records, the integrated motor core and inherited quotations remain unchanged. The older journal bullets are historical and remain superseded by the preceding augmentation: generic interactions were retained, and the GAP inference remains over-annotated rather than experimentally refuted. Source-specific UNDECIDED judgments remain explicit where the necessary target evidence is unavailable. No new GO annotation or completion credit is introduced by this follow-up.