Characterization of the alanine racemases from Pseudomonas aeruginosa PAO1.
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DadX and Alr were purified and characterised biochemically, and both complement E. coli D-alanine auxotrophs.
"The dadX(PA) and alr(PA) genes have been sequenced, overexpressed, and their activity was demonstrated by complementing d-alanine auxotrophs of Escherichia coli."
UniProt entry Q9HTQ2
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DadX racemises L-alanine to D-alanine, which DadA oxidises to pyruvate.
"Isomerizes L-alanine to D-alanine which is then oxidized to"
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PLP is covalently bound at Lys33 in the crystal structure.
"Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;"
Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Combined Automated Annotation using Multiple IEA Methods