Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
An endogenous protein inhibitor, YjhX (TopAI), for topoisomerase I from Escherichia coli.
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YjhQ is the antitoxin component of the topAI-yjhQ toxin-antitoxin system. TopAI (YjhX) inhibits topoisomerase I, causing cell death. YjhQ co-expression neutralizes TopAI toxicity. YjhQ physically interacts with TopAI in pull-down assays.
"we found a novel, previously unidentified TA system in Escherichia coli named yjhX-yjhQ. Induction of YjhX (85 amino acid residues) causes cell-growth arrest resulting in cell death, while YjhQ (181 residues) co-induction resumes cell growth"
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YjhQ binds TopAI in a pull-down assay and relieves TopAI inhibition of topoisomerase I activity in vitro.
"The addition of PrS2-YjhQ rescued topoisomerase I activity in a dose-dependent manner"
YdgG (TqsA) Controls Biofilm Formation in<i>Escherichia coli</i>K-12 through Autoinducer 2 Transport
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yjhQ was listed among genes not previously associated with biofilm formation in a YdgG/TqsA perturbation study; the cached abstract does not establish a quantitative expression ratio or direct yjhQ validation.
"proteins with unknown function (ybfG,yceO,yjhQ, andyjbE); 10 of these genes were verified through mutation"
Phosphoproteome study of Escherichia coli devoid of Ser/Thr kinase YeaG during the metabolic shift from glucose to malate.
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YjhQ is phosphorylated at Thr11 in vivo, and phosphorylation occupancy is significantly reduced in a delta-yeaG kinase mutant during glucose-to-malate shift. Suggests YjhQ participates in a kinase-regulated response to carbon-source transition.
"exemplified by phosphorylation of YjhQ at threonine 11, phosphorylation during glucose to malate shift is significantly reduced in the ΔyeaG strain compared to the wild type"
Identification of novel protein lysine acetyltransferases in Escherichia coli.
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yjhQ is present in the ASKA overexpression library (JW4269, pCA24n-yjhQ) and was included as a candidate in a systematic E. coli lysine acetyltransferase discovery screen, though no positive functional result was reported for YjhQ.
Limitations of current machine learning models in predicting enzymatic functions for uncharacterized proteins.
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DeepECTF predicted EC 2.3.1.189 (mycothiol synthase) for YjhQ, which is biologically impossible because mycothiol biosynthesis is entirely absent from E. coli. This represents a Non-Paralog Incorrect (NPI) prediction error category.
"YjhQ/b4307 is predicted to be a mycothiol synthase (EC 2.3.1.189), but mycothiol is not a molecule synthesized by E. coli, and the remaining pathway genes are absent from the genome (BioCyc ID: PWY1G-0)"