GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/aacs/aacs-uniprot.txt

UniProtKB entry for PSEPK aacs

Q88IC8 is assigned EC 6.2.1.16 and the acetoacetyl-CoA synthetase family.

PMID:7140777

Purification and characterization of acetoacetyl-CoA synthetase from Zoogloea ramigera I-16-M.

A bacterial acetoacetyl-CoA synthetase directly activates acetoacetate with ATP and CoA.

"The enzyme absolutely required ATP, CoA, a monovalent cation (K+, Rb+, Cs+ or NH+4) and a divalent cation (Mg2+, Mn2+, Ca2+ or Ni2+) for the activation of acetoacetate, yielding acetoacetyl-CoA, AMP and pyrophosphate in equimolar amounts."

PMID:10735852

Requirement for the enzymes acetoacetyl coenzyme A synthetase and poly-3-hydroxybutyrate (PHB) synthase for growth of Sinorhizobium meliloti on PHB cycle intermediates.

A bacterial homolog is required for acetoacetate activation in a PHB-cycle context.

"These findings suggest that acetoacetyl-CoA synthetase, rather than CoA transferase, activates acetoacetate to acetoacetyl-CoA in the S. meliloti poly-3-hydroxybutyrate cycle."