Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
MAML1, a human homologue of Drosophila mastermind, is a transcriptional co-activator for NOTCH receptors.
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MAML1 binds Notch intracellular domains, forms a DNA-binding complex with ICN and RBP-Jkappa, and amplifies Notch-induced HES1 transcription.
"MAML1 binds to the ankyrin repeat domain of all four mammalian NOTCH receptors, forms a DNA-binding complex with ICN and RBP-Jkappa, and amplifies NOTCH-induced transcription of HES1."
A human protein with sequence similarity to Drosophila mastermind coordinates the nuclear form of notch and a CSL protein to build a transcriptional activator complex on target promoters.
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Human MAML1 stabilizes and participates in the DNA-binding complex of intracellular Notch1 and CSL.
"hMam-1 stabilizes and participates in the DNA binding complex of the intracellular domain of human Notch1 and a CSL protein."
Identification of a family of mastermind-like transcriptional coactivators for mammalian notch receptors.
Mastermind recruits CycC:CDK8 to phosphorylate the Notch ICD and coordinate activation with turnover.
The Notch coactivator, MAML1, functions as a novel coactivator for MEF2C-mediated transcription and is required for normal myogenesis.
Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.
The notch regulator MAML1 interacts with p53 and functions as a coactivator.
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MAML1 interacts with p53 and acts as a transcriptional coactivator on p53 target genes, supporting coactivator activity but not peptide antigen binding.
"MAML1-p53 interaction involves the N-terminal region of MAML1 and the DNA-binding domain of p53, and we use a chromatin immunoprecipitation assay to show that MAML1 is part of the activator complex that binds to native p53-response elements within the promoter of the p53 target genes."
Notch signaling mediates hypoxia-induced tumor cell migration and invasion.
Direct inhibition of the NOTCH transcription factor complex.
Structural and mechanistic insights into cooperative assembly of dimeric Notch transcription complexes.
Conformational locking upon cooperative assembly of notch transcription complexes.
NOTCH1 nuclear interactome reveals key regulators of its transcriptional activity and oncogenic function.
Pharmacological disruption of the Notch transcription factor complex.
CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex.
Falcon deep research on MAML1 GO-relevant functions
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MAML1 is best modeled as a Notch transcription coactivator and complex-associated regulator rather than a generic protein-binding protein.
"MAML1 is better captured by Notch transcription coactivator/complex-associated terms than by a broad “protein binding” annotation alone."
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Notch target-gene transcription terms are more precise than broad Notch pathway terms for MAML1's direct role.
"**Prefer “Notch target gene transcription” over broad “Notch signaling pathway”** unless direct pathway-level phenotypes are tied specifically to human MAML1 (not dnMAML pan-inhibition, not paralog redundancy)."
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Nuclear speck/nuclear body annotations should be curated cautiously.
"**Nuclear bodies/specks:** nuclear-body localization is supported but is **often overexpression-linked**; annotate cautiously at specific subnuclear compartments."
NICD1 in complex with RBPJ (CSL) recruits MAML
Formation of CSL-NICD coactivator complex
NICD2 binds RBPJ and MAML in the nucleus
NOTCH1 PEST domain mutants coactivator complex binds CDK8:CCNC
NICD1 PEST domain mutants in complex with RBPJ (CSL) bind MAML
CDK8 phosphorylates NICD1 PEST domain mutants
NOTCH2 coactivator complex binds FCER2 promoter
NOTCH1 Coactivator Complex binds MYC promoter
NOTCH1 Coactivator Complex binds HES5 promoter
NOTCH1 PEST domain mutants bind HES1 promoter
NOTCH1 PEST domain mutants bind MYC promoter
NOTCH1 PEST domain mutants bind HES5 promoter
NOTCH1 PEST domain mutants bind promoters of HEY genes
RUNX3 binds the NOTCH1 coactivator complex
RUNX3:NOTCH1 coactivator complex binds the HES1 gene promoter
NOTCH1 coactivator complex binds NOTCH3 gene
NOTCH3 coactivator complex binds DLGAP5 gene promoter
NOTCH3 coactivator complex binds PBX1 gene promoter
NOTCH1,NOTCH3 coactivator complex binds PLXND1 gene promoter
NOTCH3 coactivator complex binds WWC1 (Kibra) gene promoter
MAML recruits CDK8:CCNC to xNICD1
CDK8 phosphorylates xNICD1
mNICD1 Chimeric Enhancer Complex binds Hes1 promoter
NICD3 binds RBPJ and MAML in the nucleus
Notch3 coactivator complex binds Hes1 gene promoter
NICD4 binds RBPJ and MAML in the nucleus
Notch4 binds FLT4 gene promoter