Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
UniProtKB record A0A0H2M7F4 (A0A0H2M7F4_VARPD) - Phosphate regulon transcriptional regulatory protein PhoB from Variovorax paradoxus TBEA6
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VPARA_10430 (phoB1, A0A0H2M7F4) is a 230-aa response regulator (InterPro IPR011879, NCBIfam TIGR02154, PANTHER PTHR48111:SF40) with an N-terminal receiver domain (PROSITE PS50110, residues 5-121; Asp54 phosphoacceptor) and a C-terminal OmpR/PhoB-type winged-helix DNA-binding domain (PROSITE PS51755, residues 130-226).
"DR NCBIfam; TIGR02154; PhoB; 1.
DR PANTHER; PTHR48111:SF40; PHOSPHATE REGULON TRANSCRIPTIONAL REGULATORY PROTEIN PHOB; 1."
The PhoU protein from Escherichia coli interacts with PhoR, PstB, and metals to form a phosphate-signaling complex at the membrane.
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PhoB is the winged-helix response regulator phosphorylated by PhoR under phosphate limitation to dimerize, bind Pho-box DNA upstream of Pho regulon genes, and initiate transcription.
"PhoB is a typical winged-helix response regulator that upon aspartyl phosphorylation forms a dimer, which binds to DNA sequences upstream of Pho regulon genes to recruit RNA polymerase and initiate transcription ( 7 – 10 )."