DNAH5 curation notes

Deep research

Falcon deep research (just deep-research-falcon human DNAH5) failed twice with
"Failed to authenticate" from the provider API, so no deep-research file exists.
The review is based on the cached publications and UniProt record.

Biology summary (with provenance)

• Gamma heavy chain of the outer dynein arm (ODA); orthologue of Chlamydomonas
  gamma-dynein PMID:11788826.
• Present along the whole respiratory axoneme in two ODA types PMID:18950741.
• Required for ODA assembly PMID:18950741.
• Loss causes immotile cilia PMID:15750039.
• Not in human sperm axoneme PMID:31178125; a DNAH5
  patient had normal sperm ODA heavy chains PMID:15750039.
• Cytoplasmic preassembly with ZMYND10/FKBP8 chaperone relay PMID:29916806.
• Mouse Dnah5 mutant: PCD, situs inversus, hydrocephalus PMID:11912187;
  ependymal flow PMID:15269178.

Annotation decisions

• Sperm motility IMPs (PMID:15750039, PMID:18492703) marked over-annotated: the
  first abstract contradicts a sperm role, the second rests on heterozygous
  variants.
• Cilium assembly IMP (PMID:16492982) modified to outer dynein arm assembly.
• Light intermediate chain binding (IEA/IBA) removed: the outer dynein arm has no
  light intermediate chain subunit (UniProt SUBUNIT: heavy, light and intermediate
  chains), and LIC binding is grounded only in cytoplasmic dynein 1 heavy chains
  (PTN000743491 donors DYNC1H1/Dync1h1). The claim is unlikely to be correct, not
  just under-supported, so REMOVE rather than MARK_AS_OVER_ANNOTATED.
• microtubule motor activity (IEA), axonemal dynein complex (IEA) and
  microtubule-based movement (IEA) set to TOO_GENERAL: each is entailed by a more
  specific term the review keeps (minus-end-directed microtubule motor activity,
  outer dynein arm, cilium movement).
• cilium movement involved in cell motility (IBA) modified to epithelial cilium
  movement involved in extracellular fluid movement.

PANTHER / IBA check

QuickGO IBA sets are identical for human DNAH5 (Q8TE73) and mouse Dnah5 (Q8VHE6):
7 IBA terms each, including outer dynein arm and outer dynein arm assembly from
node PTN002472868. No family-split gap of the DNAH12 kind. Note that the UniProt
PANTHER cross-reference puts DNAH5 in PTHR46532:SF13, a subfamily named after
cytoplasmic dynein 1 heavy chain 1.