Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity.
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Shieldin (REV7, SHLD1, SHLD2, SHLD3) is recruited to DSBs downstream of 53BP1-RIF1 and promotes NHEJ, class-switch recombination and fusion of unprotected telomeres.
"We propose that 53BP1-RIF1 act upstream to facilitate the recruitment of the shieldin complex, which we postulate shields DSBs against resection and thereby promotes NHEJ."
FAM35A associates with REV7 and modulates DNA damage responses of normal and BRCA1-defective cells.
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The two common FAM35A isoforms encode 904 and 835 residues. The shorter lacks a 69-residue exon from OB fold B. The 904-residue isoform 1 rescues NHEJ.
"One exon is absent in isoform 2 compared to isoform 1, deleting 69 aa from OB domain B."
ASTE1 promotes shieldin-complex-mediated DNA repair by attenuating end resection.
human/SHLD2/SHLD2-uniprot.txt
human/SHLD2/SHLD2-hpa.xml
The shieldin complex mediates 53BP1-dependent DNA repair.
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The SHLD2 C-terminal OB-fold region binds ssDNA with about 10 nM affinity. The N-terminus binds SHLD3-REV7. OB-fold mutant m1 and the short splice form SHLD2S still localise to DSBs but fail to suppress HR.
"We conclude that SHLD2 possesses ssDNA-binding activity."