Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Combined Automated Annotation using Multiple IEA Methods
MET15 as a visual selection marker for Candida albicans.
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C. albicans met15-deficient cells show delayed growth without methionine and are rescued by MET15 plasmids.
"methionine-depleted medium. However, this
growth was severely delayed."
Characterization of cell wall proteins from yeast and mycelial cells of Candida albicans by labelling with biotin: comparison with other techniques.
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The paper characterized C. albicans blastoconidial and mycelial cell-wall proteins by biotin labeling, but the cached abstract does not identify MET15.
"Labelling with biotin allowed identification of a complex array of cell wall protein and glycoprotein components within a very wide molecular mass range"
Gene disruption in Candida albicans using a synthetic, codon-optimised Cre-loxP system.
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Dennison et al. validated their Cre-loxP system by constructing C. albicans met15 null mutants.
"disrupting two C. albicans loci: ADE2 and MET15. Ade2 and met15 null mutants
were made using loxP-flanked ARG4- and HIS1-based disruption cassettes."
Mass Spectrometry-Based Proteomic and Immunoproteomic Analyses of the Candida albicans Hyphal Secretome Reveal Diagnostic Biomarker Candidates for Invasive Candidiasis.
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The study identified proteins reproducibly present in the C. albicans hyphal secretome by LC-MS/MS.
"Hyphal secreted proteins in Lee medium from S1 and S2 were in-gel digested and further analyzed by LC-MS/MS. A total of 301 proteins were identified in both replicate batches with at least 2 peptides (Table S3)."
Targeting Candida albicans O-acetyl-L-homoserine sulfhydrylase (Met15p) in antifungal treatment.
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Purified C. albicans Met15p catalyzes the PLP-dependent formation of L-homocysteine from O-acetyl-L-homoserine and sulfide.
"catalyzing formation of L-homocysteine (HCT) from O-acetyl-L-homoserine (OAH), with Na2S as a source of sulfide ion and a PLP as a cofactor"
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C. albicans Met15p did not show detectable O-acetyl-L-serine-to-L-cysteine activity under the reported assay conditions.
"no L-Cys production was detected when O-acetyl-L-serine was present in the reaction mixture instead of OAH"
Met32 governs transcriptional control of sulfur metabolic flexibility and resistance to reactive sulfur species in the human fungal pathogen Candida albicans.
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MET15 is among the C. albicans sulfur/methionine pathway transcripts induced under sulfur deprivation.
"Under sulfur deprivation, and when using Met/Cys as sulfur repletion sources, C. albicans cells induce genes involved in the different steps of methionine biosynthesis, including sulfur assimilation (MET3, MET14, MET16, MET5, MET10), incorporation into carbon chain (MET15, MET2, MET6, SAM2, MET1), and the trans-sulfuration pathway (CYS4, CYS3), which allows the interconversion between cysteine and homocysteine (Fig. 1E; Table 1)."