cyaA (PA5272, Q9HTS8) review notes

Class I adenylate cyclase (Pfam PF01295 Adenylate_cycl; IPR000274; PANTHER
PTHR38760), 950 aa, four class I domains (UniProt FT DOMAIN 21..217 "Adenylate
cyclase class-I N-terminal", second, third and fourth regions).

Literature (no deep-research provider available; own reading, 2026-11-05)

Wolfgang et al. 2003 (abstract only; Dev Cell, not in PMC): microarray study
of the TTSS regulon; mutants unable to make cAMP were profiled
PMID:12586068. The abstract does not name
cyaA; the PseudoCAP IMP rows for cyaA likely come from the cyaA cyaB double
mutant, which I could not check.
Topal et al. 2012 (full text, PMC3269522): P. aeruginosa has three ACs
PMID:22226839; CyaB dominates PMID:22226839; "As yet,
the role of P. aeruginosa CyaA is unknown."
PubMed MCP and NCBI eutils returned errors on 2026-11-05; a Europe PMC search
("cyaA" AND "cyaB" AND aeruginosa) found no CyaA-focused biochemical paper.
E. coli CyaA (P00936), the other node seed: GO:0004016 IDA PMID:6300054 and
GO:0006171 IMP PMID:4310317 (EcoCyc), checked in QuickGO 2026-11-05.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN002215143	GO:0004016 adenylate cyclase activity	2	7 (7 taxa)	GO:0004016, IMP, PMID:12586068 (PseudoCAP)	UNVERIFIABLE	Abstract only; it does not separate cyaA from cyaB. The term is right for the gene (class I catalytic domain).	E. coli CyaA P00936 (IDA PMID:6300054)	Yes; the E. coli IDA seed is sufficient and the clade is class I ACs
PTN002215143	GO:0006171 cAMP biosynthetic process	2	7 (7 taxa)	GO:0006171, IMP, PMID:12586068 (PseudoCAP)	UNVERIFIABLE	As above.	E. coli CyaA P00936 (IMP PMID:4310317)	Yes

No PSEUDOCAP or GO_CENTRAL defect: the rows are probably right, and the node
terms suit a class I AC clade. This is a different node from cyaB's
(PTN002454020, class III), so the two reviews do not share a node; both call
their PseudoCAP rows correct for the gene. The GO:0006171 IBD (dated
2026-06-16) has not yet reached this gene's GOA file.