NaODC_candidate_ODC Notes

• UniProt curates A0A314KUM9 as ornithine decarboxylase, catalyzing the PLP-dependent conversion of L-ornithine to putrescine and assigning the protein to both putrescine and nicotine biosynthesis. [file:NICAT/NaODC_candidate_ODC/NaODC_candidate_ODC-uniprot.txt "DE RecName: Full=ornithine decarboxylase"; "CC -!- CATALYTIC ACTIVITY: Reaction=L-ornithine + H(+) = putrescine + CO2"; "CC -!- PATHWAY: Alkaloid biosynthesis; nicotine biosynthesis."; "CC -!- PATHWAY: Amine and polyamine biosynthesis; putrescine biosynthesis via L-ornithine pathway; putrescine from L-ornithine: step 1/1."]
• UniProt places this candidate in plastid/chloroplast and in the class-II ornithine/lysine/arginine decarboxylase family, supporting it as a real polyamine-pathway enzyme rather than a spurious nicotine hit. [file:NICAT/NaODC_candidate_ODC/NaODC_candidate_ODC-uniprot.txt "CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast"; "CC -!- SIMILARITY: Belongs to the Orn/Lys/Arg decarboxylase class-II family."]
• The N. attenuata genome paper argues that the nicotine pathway co-opted duplicated polyamine-pathway genes, so multiple ODC-like paralogs are expected and should be curated comparatively rather than all accepted as core pathway members. PMID:28536194
• The full glucosylation preprint uses ODC, PMT, and MPO together to generate N-methylpyrrolinium for the in planta pathway reconstruction, which keeps ODC-like paralog resolution directly relevant to the minimal upstream nicotine module. [file:projects/NICOTINE_BIOSYNTHESIS/biorxiv-nicotine-glucosylation-notes.md "In N. benthamiana leaves, the in planta reconstruction uses ODC, PMT, and MPO to generate N-methylpyrrolinium, then depends on the glucosylation-late-pathway module to make labelled nicotine"; "ODC, PMT, and MPO remain part of the minimal upstream module needed to feed the completed pathway."]
• This accession is therefore a strong NaODC1/NaODC2 candidate, but resolving it against the DCOR paralog still requires evidence beyond current automated pathway annotation. [file:NICAT/NaODC_candidate_ODC/NaODC_candidate_ODC-uniprot.txt "GN Name=ODC"; "CC -!- PATHWAY: Alkaloid biosynthesis; nicotine biosynthesis."]

Description cleanup note

The YAML description field was revised to keep it as a standalone biological summary. Project-specific curation framing moved here instead.

• Moved out of the YAML description: project mapping currently treats NaODC_candidate_ODC as the stronger NICAT ornithine decarboxylase mapping for the polyamine branch that feeds nicotine biosynthesis.