Gene Ontology annotation through association of InterPro records with GO terms

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/lpdG/lpdG-uniprot.txt

UniProt record for lpdG (Q88FB1)

UniProt identifies lpdG as Dihydrolipoyl dehydrogenase (EC 1.8.1.4) and provides the seeded EC/domain/GO evidence reviewed here.

file:PSEPK/lpdG/lpdG-deep-research-asta.md

Asta deep-research retrieval for lpdG

Asta retrieval was run for this first-pass pathway curation; direct organism-specific literature was limited for several common enzyme names, so UniProt/family evidence carries the main review weight.

file:PSEPK/lpdG/lpdG-deep-research-openscientist.md

OpenScientist deep research report for lpdG

The report identifies LpdG as the KT2440 LPD-glc homolog while preserving the cross-strain evidence caveat.

PMID:1902462

Cloning and sequence analysis of the LPD-glc structural gene of Pseudomonas putida.

P. putida lpdG encodes LPD-glc, the E3 for pyruvate and 2-oxoglutarate dehydrogenases and the L-factor for glycine oxidation.

"LPD-glc, which is the E3 component of the pyruvate and 2-ketoglutarate dehydrogenase complexes and the L-factor for the glycine oxidation system"

PMID:6546487

Oxidation of glycine by Pseudomonas putida requires a specific lipoamide dehydrogenase.

Biochemical tests distinguish LPD-glc as the E3 used for P. putida glycine oxidation rather than LPD-val.

"These results indicate that LPD-glc is specifically utilized for glycine oxidation in P. putida."

file:projects/P_PUTIDA/deep-research/PSEPK__glycine_cleavage_system__ppu00260-deep-research-openscientist.md

OpenScientist PSEPK glycine cleavage system synthesis

The report surveys LpdG, Lpd, and LpdV as unresolved candidates for the shared-E3 role.

file:projects/P_PUTIDA/batches/ppu00260_glycine_cleavage_system.md

PSEPK glycine cleavage system batch

The species-specific batch identifies LpdG as the supported KT2440 L-protein candidate with an explicit strain-transfer caveat.