Gene Ontology annotation through association of InterPro records with GO terms.
Gene Ontology annotation based on Enzyme Commission mapping.
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.
Annotation inferences using phylogenetic trees
GOA UniPathway annotation
Gene Ontology annotation based on UniProtKB/Swiss-Prot KW mapping
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Automatic transfer of experimentally verified manual GO annotation data to Mus musculus orthologs using MGI orthology assertions
Combined Automated Annotation using Multiple IEA Sources.
Structural and functional characterization of the two human ThOX/Duox genes and their 5'-flanking regions.
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Functional studies show that human DUOX1 (hThOX1) and DUOX2 (hThOX2) promoters are not positively controlled by cAMP in human thyrocytes, in contrast to dog and pig thyrocytes.
"functional studies show that both hThOX promoters are not positively controlled by cAMP"
Heterodimerization controls localization of Duox-DuoxA NADPH oxidases in airway cells.
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Functional DUOX1-DUOXA1 complexes localize to the leading edge of migrating lung epithelial cells and augment cell motility and wound healing.
"Functional Duox1 and Duox2 localize to the leading edge of migrating cells, augmenting motility and wound healing."
Mammalian numb-interacting protein 1/dual oxidase maturation factor 1 directs neuronal fate in stem cells.
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Mouse Nip1/Duoxa1 interacts with Duox1 to drive Duox1-mediated ROS/H2O2 production during neural stem cell differentiation.
"The highest expression of Nip1 was observed in undifferentiated neuronal stem cells and was associated with Duox1-mediated reactive oxygen species ROS production."
When an Intramolecular Disulfide Bridge Governs the Interaction of DUOX2 with Its Partner DUOXA2.
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Mammalian DUOX enzymes act as H2O2 generators in thyroid hormone biosynthesis and mucosal innate immunity.
"As H2O2 generator, it plays a key role in both thyroid hormone biosynthesis and innate immunity."
Structures of mouse DUOX1-DUOXA1 provide mechanistic insights into enzyme activation and regulation.
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Cryo-EM structures of the mouse DUOX1-DUOXA1 complex in NADPH-free, NADPH-bound, and dimer-of-dimer states reveal the mechanism of DUOX1-DUOXA1 assembly, cofactor binding, and H2O2 production.
"Here I report cryo-EM structures of mouse DUOX1–DUOXA1 complexes in NADPH-free, NADPH-bound and dimer-of-dimer states, providing structural mechanisms underlying DUOX1–DUOXA1 activation and regulation and molecular insights into ROS production."
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The N-terminal peroxidase-homology domain (PHD) of mouse DUOX1 lacks heme and lacks the histidine residues that coordinate heme in genuine peroxidases.
"Though the PHD of DUOX1 has an overall architecture of peroxidase with an RMSD of 1.3 Å when superimposed with the bovine lactoperoxidase (LPO, PDB 5B72) (Extended Data Fig. 2a)21, the facts that no heme density is observed in the cryo-EM map and histidines needed for heme coordination are missing in the putative binding pocket strongly suggest that the PHD domain of DUOX1 lacks heme-dependent peroxidase activity."
Dual oxidase 1 promotes antiviral innate immunity.
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Duox1 promotes antiviral innate immunity in the mouse airways in vivo, reducing influenza A virus mortality, morbidity, and lung viral load.
"Here, we show that Duox1 promotes antiviral innate immunity in vivo. Upon influenza airway challenge, Duox1-/- mice have enhanced mortality, morbidity, and impaired lung viral clearance."
UniProtKB flat file for human DUOX1 (Q9NRD9)
UniProtKB flat file for mouse Duox1 (A2AQ92)