Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
The human plasma proteome: a nonredundant list developed by combination of four separate sources.
TSG-6 potentiates the antitissue kallikrein activity of inter-alpha-inhibitor through bikunin release.
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Free bikunin, rather than HC-associated bikunin, provides relevant protease inhibition.
"bikunin in its free state, but not when associated with HCs, is a relevant protease inhibitor"
The TSG-6/HC2-mediated transfer is a dynamic process shuffling heavy chains between glycosaminoglycans.
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Bikunin-complex heavy chains can be transferred to hyaluronan.
"can be transferred to different hyaluronan (HA) molecules by TSG-6/HC2"
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HA-linked heavy chains can serve as donors in subsequent reactions.
"HCs transferred to HA may function as HC donors in subsequent transfer reactions"
Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
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The HDA source analyzed pooled EPS-urine exosome preparations.
"In pooled EPS-urine exosome samples, ~900 proteins were detected."
Isolation and characterization of cDNAs encoding the heavy chain of human inter-alpha-trypsin inhibitor (I alpha TI): unambiguous evidence for multipolypeptide chain structure of I alpha TI.
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The historical calcium claim was a sequence-based possibility, not a binding assay.
"The H chain contains potential calcium-binding sites"
Analysis of inter-alpha-trypsin inhibitor and a novel trypsin inhibitor, pre-alpha-trypsin inhibitor, from human plasma. Polypeptide chain stoichiometry and assembly by glycan.
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The inter-alpha-inhibitor heavy chains are noninhibitory.
"Inter-alpha-trypsin inhibitor contains noninhibitory heavy chains of 65,000 and 70,000 Da"
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A distinct 30-kDa chain supplies trypsin-inhibitory activity.
"each protein contained a single, identical, trypsin-inhibitory chain of 30,000 Da"
Extracellular matrix signatures of human primary metastatic colon cancers and their metastases to liver.
Metal Ion-dependent Heavy Chain Transfer Activity of TSG-6 Mediates Assembly of the Cumulus-Oocyte Matrix.
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The human HC1 VWFA domain contains a magnesium-bearing MIDAS motif.
"its von Willebrand factor A domain contains a Mg2+-containing MIDAS motif"
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Loss of the coordinating HC1 aspartate abolishes metal binding.
"mutation of the chelating Asp-298 residue abolishes metal ion binding"
Characterization of the Extracellular Matrix of Normal and Diseased Tissues Using Proteomics.
A serum-derived hyaluronan-associated protein (SHAP) is the heavy chain of the inter alpha-trypsin inhibitor.
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Human serum-derived HA-associated proteins include HC1.
"essentially 100% with human SHAP"
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Mapping implicated the C-terminal half of the heavy chain in HA association.
"participation of the COOH-terminal half of ITI with an amphipathic alpha helix structure in the HA binding"
Chondroitin sulphate covalently cross-links the three polypeptide chains of inter-alpha-trypsin inhibitor.
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Chondroitin sulfate covalently assembles bikunin with HC1 and HC2.
"the different peptide chains constituting ITI are associated by the new cross-link described as the protein-glycosaminoglycan-protein cross-link"
UniProtKB record for human ITIH1 (P19827)
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ITIH1 is a reviewed secreted precursor.
"SUBCELLULAR LOCATION: Secreted."
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HC1 is ester-linked to bikunin chondroitin sulfate.
"Heavy chains are linked to bikunin via chondroitin 4-sulfate"
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UniProt maps a VWFA domain and an HA-associated region.
"Hyaluronan-binding"
Manual literature and annotation review notes for human ITIH1
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Protease inhibition must not be transferred from the complex to HC1.
"ITIH1 must not inherit the serine-protease inhibitor activity of the assembled complex."
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The calcium annotation is replaced by direct magnesium evidence.
"Modern direct evidence instead supports magnesium ion binding"
Manual literature synthesis for human ITIH1 (P19827)
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HC1 is a transferable HA-associated heavy chain, not an inhibitor or transferase.
"HC1 is therefore best understood as a transferable extracellular heavy-chain component that decorates and helps organize HA matrices"