Gene Ontology annotation through association of InterPro records with GO terms

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000118

TreeGrafter-generated GO annotations

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/lpdV/lpdV-uniprot.txt

UniProt record for lpdV (Q88EP9)

UniProt identifies lpdV as Dihydrolipoyl dehydrogenase (EC 1.8.1.4) and provides the seeded EC/domain/GO evidence reviewed here.

file:PSEPK/lpdV/lpdV-deep-research-asta.md

Asta deep-research retrieval for lpdV

Asta retrieval was run for this first-pass pathway curation; direct organism-specific literature was limited for several common enzyme names, so UniProt/family evidence carries the main review weight.

file:PSEPK/lpdV/lpdV-deep-research-openscientist.md

OpenScientist deep research report for lpdV

The report identifies LpdV as the bkd-operon LPD-val enzyme of the branched-chain 2-oxoacid dehydrogenase complex.

PMID:1902462

Cloning and sequence analysis of the LPD-glc structural gene of Pseudomonas putida.

P. putida LPD-val is the specific E3 of the branched-chain keto acid dehydrogenase complex.

"LPD-val, which is the specific E3 component of the branched-chain keto acid dehydrogenase complex"

PMID:6546487

Oxidation of glycine by Pseudomonas putida requires a specific lipoamide dehydrogenase.

P. putida glycine oxidation uses LPD-glc rather than LPD-val.

"The partially purified glycine oxidation system from P. putida was stimulated by LPD-glc but not by LPD-val"

file:projects/P_PUTIDA/batches/ppu00260_glycine_cleavage_system.md

PSEPK glycine cleavage system batch

LpdV is excluded from the physiological GCS L role because it is the bkd-linked LPD-val paralog.