Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Unveiling the genes responsible for the unique Pseudomonas aeruginosa oleate-diol synthase activity.
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PA2078 encodes the 7,10-diol synthase that converts 10-H(P)OME into 7,10-DiHOME.
"whereas PA2078 encodes for the (7S,10S)-hydroperoxide diol synthase enzyme (7,10-DS) which allows the conversion of 10-H(P)OME into 7,10-DiHOME."
In Silico/In Vivo Insights into the Functional and Evolutionary Pathway of Pseudomonas aeruginosa Oleate-Diol Synthase. Discovery of a New Bacterial Di-Heme Cytochrome C Peroxidase Subfamily.
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Neither heme site of PA2078 is needed for its diol synthase activity.
"These results indicate that neither the proximal or distal heme groups, nor the cysteine putatively related to P450 enzymes are involved in the catalytic activity of PA2078."
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PA2077 and PA2078 are periplasmic.
"both proteins display type I signal export sequences that allow transport of the synthesized products to the periplasm, a fact experimentally confirmed by PhoA fusion screening"
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Co-expression of PA2078 abolishes the toxicity of PA2077.
"Normal growth rates and full activity were achieved in both mutants, indicating that when the two enzymes are co-expressed in trans, both maintain their respective functions, with PA2078 abolishing toxicity."