pppA (PA0075, Q9I757) review notes

Literature

Search (Europe PMC REST, 2026-11-05: TITLE:PppA AND aeruginosa) returned
PMID:31208722 (crystal structure) and PMID:17085567 (an unrelated E. coli yghJ-pppA
prepilin peptidase gene, a symbol collision, not used). The PubMed search API was
returning errors that day.

  1. Mougous et al. 2007 PMID:17558395, abstract only (no PMC copy). PpkA is required for
    H1-T6SS assembly and Hcp1 secretion PMID:17558395;
    PppA antagonises it PMID:17558395; both act on Fha1.
  2. Wu et al. 2019 PMID:31208722, abstract only. 2.10 A crystal structure (PDB 6JKV):
    PPM core, flexible flap, two Mn ions PMID:31208722.
  3. UniProt Q9I757: PPM-type phosphatase domain 8-241 (PROSITE PS51746), PANTHER PTHR13832
    (PP2C), Mn-binding residues from PDB 6JKV.

PseudoCAP IBA-seed assessment

PTN node node term n_seeds leaf IBAs seed annotation (term, evidence, PMID) verdict rationale other seeds IBD stands without this seed?
PTN000352745 GO:0004722 protein serine/threonine phosphatase activity 35 1913 (121 taxa) GO:0004722, IDA, PMID:17558395 (PseudoCAP) SOUND Abstract names PppA as the Ser-Thr phosphatase antagonising PpkA on Fha1; PPM fold and dinuclear Mn centre (PMID:31208722) fit the activity. Abstract-only reading, but no defect is claimed. 34 others: yeast PTC1-7, S. pombe ptc1-4, Arabidopsis PP2Cs (ABI1-like etc.), human/mouse/rat PPM1 family, M. tuberculosis PstP (P9WHW5), Plasmodium, Dictyostelium, C. elegans FEM-2 Yes; the node is overwhelmingly seeded by characterised PP2Cs. The node term suits a PP2C clade. No GO_CENTRAL defect.

The signal transduction IBD at the same node is seeded by plant/mammalian PP2Cs, not by
PppA; it is appropriate for PppA as well.

Annotation decisions