Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Expression of Pseudomonas aeruginosa virulence genes requires cell-to-cell communication.
-
lasI is required for high-level elastase expression and for synthesis of the diffusible autoinducer PAI.
"The lasl gene is involved in the synthesis of a diffusible molecule termed Pseudomonas autoinducer (PAI)."
Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes.
-
The autoinducer made by P. aeruginosa, or by E. coli carrying lasI, is 3-oxo-C12-HSL.
"Chemical analyses indicated the purified material was 3-oxo-N-(tetrahydro-2-oxo-3-furanyl)dodecanamide."
Beta-ketoacyl acyl carrier protein reductase (FabG) activity of the fatty acid biosynthetic pathway is a determining factor of 3-oxo-homoserine lactone acyl chain lengths.
-
A reconstituted system with purified Fab enzymes, ACP and LasI makes almost only 3-oxo-C12-HSL.
"In the presence of excess protein, substrates and cofactors, this system produced almost exclusively 3-oxo-C(12)-HSL."
Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI.
-
Crystal structure of LasI; acyl-chain tunnel and acyl-ACP binding surface (Arg154, Lys150).
"Analysis of AHL-synthase surface residues identified a binding site for acyl-ACP, a role that was supported by in vivo reporter assay analysis of the mutated residues, including Arg154 and Lys150."
UniProt entry P33883 (LASI_PSEAE)