Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase.
Characterization of recombinant human nicotinamide mononucleotide adenylyl transferase (NMNAT), a nuclear enzyme essential for NAD synthesis.
Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms.
Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis.
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Detailed initial-rate kinetics of NMNAT1 defining substrate specificity (NMN and NaMN), ordered ATP-before-NMN mechanism, metal-ion preference and product inhibition; basis for both EC 2.7.7.1 and EC 2.7.7.18 assignments.
Enzymes in the NAD+ salvage pathway regulate SIRT1 activity at target gene promoters.
Next-generation sequencing to generate interactome datasets.
Proteomic characterization of the human sperm nucleus.
Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
A proteome-scale map of the human interactome network.
A massively parallel pipeline to clone DNA variants and examine molecular phenotypes of human disease mutations.
Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
ADP-ribose-derived nuclear ATP synthesis by NUDIX5 is required for chromatin remodeling.
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Nuclear ATP is generated from hydrolysis of poly(ADP-ribose) to ADP-ribose, which NUDIX5 (with pyrophosphate) converts to ATP; this nuclear ATP source is essential for hormone-induced chromatin remodeling. NMNAT1 acts in this nuclear ATP-generating module together with PARP1, PARG and NUDT5.
Architecture of the human interactome defines protein communities and disease networks.
Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.
A reference map of the human binary protein interactome.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
NMNAT1 transfers an adenylyl group from ATP to NAMN to yield NAAD