Gene Ontology annotation through association of InterPro records with GO terms

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity

GO_REF:0000033

Annotation inferences using phylogenetic trees

GO_REF:0000107

Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

PMID:21516116

Next-generation sequencing to generate interactome datasets.

The exact CHN1–NCK2 yeast two-hybrid source record supports assay binding.

PMID:25416956

A proteome-scale map of the human interactome network.

Sequence-confirmed human ORF interactions support NCK2 and HEMK1 binding capability in the original screen.

PMID:25910212

Widespread macromolecular interaction perturbations in human genetic disorders.

Matched wild-type/mutant screening preserves the CHN1–NCK2 pair and its tested allele scope.

PMID:31515488

Extensive disruption of protein interactions by genetic variants across the allele frequency spectrum in human populations.

Variant-sensitive Y2H measurements support the wild-type interaction context without declaring disruptive alleles functional.

PMID:32296183

A reference map of the human binary protein interactome.

Human ORF screens identify CHN1 interactions with NCK2, MAPK1, ANKK1 and HEMK1.

Reactome:R-HSA-9012999

RHO GTPase cycle

CHN1 is explicitly represented among cytosolic RAC1 GAPs within the RHO GTPase cycle.

Reactome:R-HSA-9013144

RAC1 GAPs stimulate RAC1 GTPase activity

CHN1 is explicitly represented among cytosolic RAC1 GAPs within the RHO GTPase cycle.

PMID:8336731

Alpha 2-chimerin, an SH2-containing GTPase-activating protein for the ras-related protein p21rac derived by alternate splicing of the human n-chimerin gene, is selectively expressed in brain regions and testes.

Purified human alpha2 stimulates Rac GTP hydrolysis; Rho and CDC42Hs are ineffective substrates under the tested conditions.

PMID:18653847

Human CHN1 mutations hyperactivate alpha2-chimaerin and cause Duane's retraction syndrome.

Human alpha2 lowers cellular Rac-GTP; disease variants increase the effect, and alpha2 overexpression itself can disrupt axon growth.

PMID:17785182

EphA4-dependent axon guidance is mediated by the RacGAP alpha2-chimaerin.

Alpha-chimaerin associates with Eph/NCK proteins and participates in Eph-linked growth-cone signaling; receptor coexpression does not uniformly increase GAP activity.

PMID:17785183

alpha2-Chimaerin is an essential EphA4 effector in the assembly of neuronal locomotor circuits.

Mouse alpha2 is an EphA4-associated signaling effector required for growth-cone responses and cortical/spinal circuit guidance.

PMID:17719550

Rac-GAP alpha-chimerin regulates motor-circuit formation as a key mediator of EphrinB3/EphA4 forward signaling.

The original source links alpha-chimaerin to EphA4, Rac and corticospinal guidance in the miffy mouse model.

PMID:25673830

α2-chimaerin is required for Eph receptor-class-specific spinal motor axon guidance and coordinate activation of antagonistic muscles.

Alpha2 GAP signaling supports EphA-selective lateral motor-axon guidance despite association with both EphA4 and EphB1.

file:human/CHN1/CHN1-source-evidence.json

CHN1 source-linked GAP, Eph, interaction and propagation evidence

PMID:18826946

Identification of an autoinhibitory mechanism that restricts C1 domain-mediated activation of the Rac-GAP alpha2-chimaerin.

Human alpha2 C1-dependent DAG recognition supports EGF-induced membrane recruitment, with autoinhibition and additional inputs limiting activation.

PMID:7518459

Close similarity of baculovirus-expressed n-chimaerin and protein kinase C alpha as phorbol ester receptors.

Recombinant n-chimaerin displays high-affinity phorbol-ester binding in the reported in-vitro assay.