Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Probing the diversity of the Arabidopsis glutathione S-transferase gene family.
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Survey of the 47-member Arabidopsis GST superfamily defining the plant-specific phi and tau classes; family-level statements on detoxification.
"the large phi (GSTF) and tau (GSTU) classes are plant specific"
Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome A-mediated signaling in Arabidopsis.
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FIP1 (GSTU20) identified as a FIN219 interactor; interaction confirmed in vitro and in vivo.
"we demonstrate that FIN219-interacting protein 1 (FIP1) interacts with FIN219 in vitro and in vivo"
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Recombinant FIP1 conjugates GSH to CDNB with measured Km values.
"The result showed that FIP1 can use GSH and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates"
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GUS-FIP1 localises to cytoplasm and nucleus in onion cells.
"bombarded cells showed the GUS-FIP1 fusion protein localized in the cytoplasm and the nucleus"
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Altered FIP1 dosage causes far-red hyposensitivity and late flowering.
"The gain of function and partial loss of function of FIP1 resulted in a hyposensitive hypocotyl phenotype under continuous FR (cFR) light"
Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis.
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GSTU20 (At1g78370) and GSTF11 (At3g03190) are regulated by MYB28 together with the aliphatic glucosinolate pathway genes.
"Transcriptome analyses of myb28 and Myb28 -overexpressing cell cultures indicated that both of them are regulated by Myb28"
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics; an oxidative stress case study.
Enzyme activities and subcellular localization of members of the Arabidopsis glutathione transferase superfamily.
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Systematic assay of recombinant Arabidopsis phi and tau GSTs with CDNB, BITC and cumene hydroperoxide; BITC (a glucosinolate breakdown product) is a preferred substrate of tau GSTs.
"BITC was a more discriminating GST substrate, being acted on by most tau class enzymes but rarely by the GSTFs."
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Notes the proteomic detection of GSTU20 in chloroplast preparations.
"GSTs F2, F8, F9, F10, U19, U20, L2, and DHAR3 in the chloroplast"
A proteomics approach identifies novel proteins involved in gravitropic signal transduction.
A proteomic strategy for global analysis of plant protein complexes.
Structural basis of jasmonate-amido synthetase FIN219 in complex with glutathione S-transferase FIP1 during the JA signal regulation.
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FIP1 has a canonical homodimeric GST fold with GSH bound in the G-site.
"FIP1 possesses a canonical, homodimerized GST protein fold."
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FIP1 binding roughly doubles FIN219 adenylation activity.
"the adenylation activity per mole of FIN219–FIP1 was approximately double the adenylation activity per mole of FIN219 alone"
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
Proteome analysis of peroxisomes from dark-treated senescent Arabidopsis leaves.
Glutathione Transferase U13 Functions in Pathogen-Triggered Glucosinolate Metabolism.
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Comparator for the NEW-term decision. GSTU13 is required for the PEN2 indole glucosinolate pathway and carries a TAIR IMP annotation to indole glucosinolate catabolic process (acts_upstream_of_or_within), a precedent for mutant-based glucosinolate process annotations on GSTs.
"Glutathione-S-Transferase class-tau member 13 (GSTU13) as an indispensable component of the PEN2 immune pathway for IG metabolism"
Arabidopsis Glutathione-S-Transferases GSTF11 and GSTU20 Function in Aliphatic Glucosinolate Biosynthesis.
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CRISPR gstu20 and gstf11 knockouts reduce almost all aliphatic glucosinolates in leaves and seeds, with no effect on indolic glucosinolates; gstu20 is stronger.
"both gstf11 and gstu20 mutants exhibited substantial reduction in almost all categories of aliphatic GSLs with different side chain lengths"
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The double mutant is additive but not null, implying other GSTs or non-enzymatic conjugation contribute.
"the double mutation of GSTF11 and GSTU20 caused a dramatic decrease but did not completely abolish the formation of aliphatic GSLs"
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Enzyme activity on the native intermediate was not tested; authors call for validation.
"the levels of protein and its derived enzymatic activity should be validated in future studies"
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gstu20 mutation causes broad transcriptome changes (1,232 DEGs).
"In gstu20, 1,232 genes were identified as DEGs"