mucR (PA1727, Q9I310) review notes

Review session 2026-11-05 (PseudoCAP IBA-seed protocol, tier 2)

Fetched with just fetch-gene PSEAE Q9I310 --alias mucR; accession matches the worklist.
No deep-research provider used. PMID:23729646 and PMID:16477007 are abstract-only (PubMed MCP
returned no body for PMC3754559); PMID:19088322 and PMID:28186120 have cached full text.

Literature

MucR is an inner-membrane MHYT-GGDEF-EAL protein; LacZ/PhoA fusions place the GGDEF/EAL
C-terminus in the cytosol PMID:19088322
MucR drives alginate production through a localized c-di-GMP pool near Alg44; deletion of the
GGDEF domain abolishes function PMID:19088322
Bifunctional in vitro PMID:23729646
Dispersion phenotype in response to NO and glutamate PMID:23729646. Unlike NbdA, MucR is not shown to be NO-induced; the NO-response row is a phenotype shared with another cue.
Crystal structures of the MucR EAL domain with c-di-GMP and Mg2+ PMID:28186120

Annotation decisions

ACCEPT: GO:0052621 (IDA x2, IEA), GO:0071111 (IDA, IBA, IEA), GO:0005886 (EXP, IEA).
KEEP_AS_NON_CORE: GO:0071732 cellular response to nitric oxide (IMP, IEA/ARBA). Kept consistent
with nbdA, where the same term is ACCEPTED because NbdA is NO-induced and NO-specific.
Action counts: ACCEPT 8, KEEP_AS_NON_CORE 2. No source defects.

PseudoCAP IBA-seed assessment

Shares PTN002403463 with PA5017 (DipA, Q9HUF2); same verdict and no node-level defect (see
PA5017 notes, which also record the EAL-less PA4929 leaf as a leaf-level exception). This node
is separate from PTN002105365 (rocR review) and PTN002895351 (wspR review).

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN002403463	GO:0071111 cyclic-guanylate-specific phosphodiesterase activity	3	14 (5 taxa)	GO:0071111, IDA, PMID:23729646 (PseudoCAP)	SOUND	Abstract states outright that MucR displayed PDE activity in vitro; the EAL domain binds c-di-GMP/Mg2+ in crystal structures (PMID:28186120).	E. coli PdeR P77334 (IDA PMID:17010156, EXP PMID:23708798), PA5017 Q9HUF2 (IDA PMID:22493016)	Yes