ANKRD46 is consistently identified as a 3' UTR-targeted effector gene regulated by oncogenic and tissue-context miRNAs, rather than through any defined intrinsic biochemical activity [PMID:21219636, PMID:25542822]. It is a direct target of miR-21, which binds its 3' UTR and represses its expression in breast cancer cells, such that miR-21 knockdown increases ANKRD46 protein PMID:21219636. In mouse luminal epithelium, Ankrd46 is repressed by miR-451 in a manner implicated in embryo implantation PMID:25542822. The intrinsic molecular function and biochemical activity of the ANKRD46 protein have not been characterized in the available corpus; all available evidence positions it as a downstream node of miRNA-mediated regulation across cancer and reproductive tissues.
| Year | Confidence | Finding | PMIDs | Journal |
|---|---|---|---|---|
| 2011 | Medium | ANKRD46 is a direct transcriptional target of miR-21; miR-21 binds the 3' UTR of ANKRD46 and suppresses its expression, as demonstrated by luciferase reporter assay and Western blot showing increased ANKRD46 protein upon miR-21 knockdown in breast cancer cells. | PMID:21219636 | Breast cancer research : BCR |
| 2014 | Medium | Ankrd46 is a direct target of miR-451 in mouse luminal epithelium; miR-451 binds and represses Ankrd46, and this interaction is implicated in embryo implantation, as shown by dual-luciferase assay and loss-of-function/gain-of-function experiments in vivo. | PMID:25542822 | Fertility and sterility |
| 2014 | Low | ANKRD46 is a validated miR-21 target in hepatocellular carcinoma cells; uptake of anti-miR-21 induces ANKRD46 expression and inhibits cell growth, confirming miR-21-mediated repression of ANKRD46 in this context. | PMID:25550434 | Nucleic acids research |
| 2023 | Low | ANKRD46 expression is associated with the IL6-JAK-STAT3 signaling pathway in keloid and T2DM fibrotic contexts, and miR-21 regulates ANKRD46 expression within the fibrotic microenvironment; validated by Western blotting, qRT-PCR, IHC, and flow cytometry in mouse models. | PMID:41346282 | International journal of surgery (London, England) |
| 2023 | Low | Circ_PRDM5 acts as a sponge for miR-25-3p, which in turn targets ANKRD46; circ_PRDM5 overexpression induces ANKRD46 upregulation via miR-25-3p sponging, and inhibition of miR-25-3p increases ANKRD46 levels and retards breast cancer progression, establishing ANKRD46 as a downstream effector of the circ_PRDM5/miR-25-3p axis. | PMID:37485755 | Journal of biochemical and molecular toxicology |