Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Molecular interactions of N-RAP, a nebulin-related protein of striated muscle myotendon junctions and intercalated disks.
Conformation, localization, and integrin binding of talin depend on its interaction with phosphoinositides.
-
PI(4,5)P2 binding activates talin and enhances integrin binding.
"the interaction between integrin and talin was greatly enhanced by PI4,5P(2)-induced talin activation"
Disruption of focal adhesions by integrin cytoplasmic domain-associated protein-1 alpha.
Structural basis for amplifying vinculin activation by talin.
-
All three talin VBSs bind and activate the vinculin head by helical bundle conversion.
"all of talin VBSs activate vinculin by provoking helical bundle conversion of the Vh domain, which displaces the vinculin tail (Vt) domain"
Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion.
A quantitative protein interaction network for the ErbB receptors using protein microarrays.
TA205, an anti-talin monoclonal antibody, inhibits integrin-talin interaction.
Identification of a repeated domain within mammalian alpha-synemin that interacts directly with talin.
Large-scale proteomics and phosphoproteomics of urinary exosomes.
ANKRD1, the gene encoding cardiac ankyrin repeat protein, is a novel dilated cardiomyopathy gene.
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Protein profile of exosomes from trabecular meshwork cells.
Analysis of the myosin-II-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation.
Platelet proteome analysis reveals integrin-dependent aggregation defects in patients with myelodysplastic syndromes.
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Dual role of vinculin in barrier-disruptive and barrier-enhancing endothelial cell responses.
THSD1 (Thrombospondin Type 1 Domain Containing Protein 1) Mutation in the Pathogenesis of Intracranial Aneurysm and Subarachnoid Hemorrhage.
Systems analysis of RhoGEF and RhoGAP regulatory proteins reveals spatially organized RAC1 signalling from integrin adhesions.
Proteome-scale mapping of binding sites in the unstructured regions of the human proteome.
Proteome-scale characterisation of motif-based interactome rewiring by disease mutations.
Translocation of PTK2 to Focal complexes
Autophosphorylation of PTK2 at Y397
Integrin alphaIIb beta3 activation
Recruitment of GRB2 to p-PTK2
Phosphorylation of pPTK2 by SRC
Interaction of integrin alphaIIb beta3 with Fibrinogen
Interaction of SOS with GRB2 bound to FADK1
Phosphorylation of BCAR1 by SRC-PTK2 complex
Recruitment of BCAR1 to PTK2 complex
Autophosphorylation of SRC
Clustering of Integrin alphaIIb beta3 complexes
Dephosphorylation of inactive SRC by PTPB1
Inhibition of integrin activation by sequestering PIP5KIgamma
SYK binds to integrin alphaIIb beta3
Activated integrin alphaIIb beta3 binds SHC1
SHC1 bound to integrin alphaIIb beta3 is phosphorylated somehow
SHC1 dissociates from integrin alphaIIb beta3
Release Of ADP From Myosin
Release of platelet cytosolic components
Ancient origin of the integrin-mediated adhesion and signaling machinery.
-
Talin, vinculin, paxillin and alpha-actinin are found across unikonts (opisthokonts plus amoebozoans), predating animals; they were later co-opted into the integrin adhesome.
"Many of the scaffolding proteins (talin, vinculin, paxillin) most likely evolved in the common ancestor of amoebozoans and opisthokonts, where they had ancestrally different functions"
Analysis of a vinculin homolog in a sponge (phylum Porifera) reveals that vertebrate-like cell adhesions emerged early in animal evolution.
-
A sponge talin vinculin-binding-site peptide binds sponge vinculin and activates its F-actin binding.
"In the presence of Op talin peptide, full-length Op vinculin bound to F-actin filaments"
Integrin-Mediated Adhesion in the Unicellular Holozoan Capsaspora owczarzaki.
-
Integrin beta2 and vinculin localize to adhesive filopodia of Capsaspora; talin itself was not examined in the abstract.
"We show that integrin β2 and its associated protein vinculin localize as distinct patches in the filopodia."