Use of the ND evidence code for Gene Ontology (GO) terms

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

GO_REF:0000122

AtSubP analysis

PMID:11752391

A genome-wide analysis of Arabidopsis Rop-interactive CRIB motif-containing proteins that act as Rop GTPase targets.

RIC7 interacts with active ROP1, associates with pollen-tube plasma membrane and cytoplasm, and affects tube elongation when expressed in tobacco pollen.

PMID:18178769

The Arabidopsis small G protein ROP2 is activated by light in guard cells and inhibits light-induced stomatal opening.

RIC7 binds active ROP2 and undergoes light- and ROP-dependent redistribution in guard cells.

file:ARATH/Q1G3K8/Q1G3K8-uniprot.txt

UniProt Q1G3K8: CRIB domain-containing protein RIC7 (AT4G28556)

The 216-residue protein contains a CRIB domain and interacts with ROP1 and ROP2.

PMID:26451971

The ROP2-RIC7 pathway negatively regulates light-induced stomatal opening by inhibiting exocyst subunit Exo70B1 in Arabidopsis.

RIC7 restrains light-induced stomatal opening in Arabidopsis.

"Light-induced stomatal opening was promoted by ric7 knockout, whereas it was inhibited by RIC7 overexpression"

file:ARATH/Q1G3K8/Q1G3K8-bioinformatics/RESULTS.md

RIC7 sequence identity and experimental primer mapping

Published RIC7 primers from Wu and Hong match the AT4G28556 transcript, and Q1G3K8 matches its RefSeq protein and DQ487576 CDS translation.

PMID:33586611

RIC7 plays a negative role in ABA-induced stomatal closure by inhibiting H(2)O(2) production.

RIC7 overexpression attenuates ABA-induced stomatal closure and is associated with lower guard-cell ROS accumulation.

"using two RIC7 overexpressing mutants, we confirmed the negative role of RIC7 in ABA-induced stomatal closure"