FY (At5g13480, UniProt Q6NLV4) curation notes

2026-10 review session (autonomous pathway module)

• Accession verified: Q6NLV4 FY_ARATH, At5g13480.
• Deep research (falcon) failed (HTTP 429).
• FY = Pfs2p/WDR33 ortholog PMID:12809608
• Stable CPSF association; transient FCA PMID:19439664
• Null alleles embryo lethal; PPLPP motifs bind FCA PMID:16033802
• SDG26 links FY to FLD complex PMID:32541063

Decisions

• CPSF complex (IBA) and mRNA 3'-end processing (IEA) accepted as core.
• Six bare protein-binding rows removed (CPSF membership captured by GO:0005847).
• CRL4 complex (DDB1 interaction of DWD protein) kept as non-core: no functional evidence.
• NEW: mRNA alternative polyadenylation (GO:0110104).
• No MF assigned in core_functions: direct RNA (PAS) binding by plant FY has not been demonstrated.

2026-10-26 autonomous-flowering RNA-factor audit

All rows were reviewed, preserving original terms, evidence, references, qualifiers, NOT flags and supporting entities. Falcon/fallback research and gene-publication retrieval ran concurrently under the parent workflow; provider attempts failed and logs remain under .cache/module-audit-logs/autonomous-flowering-fetch/. No provider report was fabricated. Primary source caches and curated UniProt evidence were inspected independently. Annotation-reviewer consultation with the chromatin-panel reviewer agreed that nuclear predominance is not organelle exclusion, broad RNP membership does not require PABP identity, and FPA termination reinterpretations are locus-specific.

The authoritative PAINT inspection is reproducible in genes/ARATH/FCA/FCA-bioinformatics/. It uses exact existing PTNs and is not a reconstructed family phylogeny. The failed full-family download was not used; only its incomplete .part file was removed after disk exhaustion. Current source retrieval is recorded honestly: PMID:18223036 refreshed complete; PMID:19439664 cache abstract-only but full web text inspected; 16820865 and PMID:19561057 recovered normally; optional PMID:16753815 fetch returned 429 and was not used to support a new claim.

• Refreshed PMID:18223036 contains complete Results/Methods. Figure 4A explicitly retrieves FY with DDB1b together with CUL4. Retain CRL4-complex association as contextual non-core; Figure 4B CUL4 bait and AKIN10 degradation are PRL1 experiments and do not demonstrate FY substrate selection.
• PMID:19439664 cache remains abstract-only after normal PMC fallbacks. Complete online PMC text inspected at https://pmc.ncbi.nlm.nih.gov/articles/PMC2689023/ (2026-10-26). Native stable FY/CPSF100/CPSF160 assemblies are supported; FCA was not stably co-purified. Do not claim the cached file contains the complete paper.
• Exact PTN000519834 CPSF IBD independently matches native complex purification. No direct plant FY RNA-binding/nuclease activity is added from WDR33 analogy. Existing NEW APA retained.

PR #5574 prose and justification follow-up

Corrected citation spacing in reviewer-authored prose without altering original annotation payloads, source quotations, raw source files or prior histories. Biological decisions, annotation actions and source-access limits are unchanged.