ILV1 (YER086W, P00927) notes

• Biosynthetic threonine dehydratase EC 4.3.1.19, mitochondrial [UniProt:P00927 "RecName: Full=Threonine dehydratase, mitochondrial;"]; reaction [UniProt:P00927 "Reaction=L-threonine = 2-oxobutanoate + NH4(+)"].
• Allosteric regulation [UniProt:P00927 "ACTIVITY REGULATION: Isoleucine allosterically inhibits while valine"]; PMID:7042346.
• Location: mitochondrion by GFP (PMID:14562095) and proteomics (PMID:14576278, 16823961, 24769239) [UniProt:P00927 "SUBCELLULAR LOCATION: Mitochondrion"].
• Classical genetics: is-1 mutant with altered threonine deaminase (PMID:5345980; title-only cache).
• Relationship with CHA1: PMID:7042346; the two PMID:7042346.

Curation observations

• RCA cytosol rows (2) contradict mitochondrial location -> REMOVE.
• IGI L-threonine catabolic process (with CHA1) kept as non-core (backup).
• Module: Ilv1 is the yeast biosynthetic IlvA-family entry enzyme; Cha1 is a catabolic paralog in the same PANTHER family (PTHR48078 SF2 vs SF11) and should not satisfy the biosynthetic step except as a minor backup.