GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

InterPro2GO maps the GH1 family signature to carbohydrate metabolic process; modified to glucosinolate catabolic process since the physiological substrates are thioglucosides.

GO_REF:0000003

Gene Ontology annotation based on Enzyme Commission mapping

EC-number mapping yields thioglucosidase (EC 3.2.1.147; core) and beta-glucosidase (EC 3.2.1.21; weak, non-core) activities.

GO_REF:0000033

Annotation inferences using phylogenetic trees

PAINT propagates beta-glucosidase activity from the GH1 node (retained weakly; non-core) and glucosinolate catabolic process from the myrosinase node (accepted).

GO_REF:0000120

Combined Automated Annotation using Multiple IEA Methods

Combined IEA assigns O-glycosyl hydrolase activity, the parent of thioglucosidase activity.

GO_REF:0000122

AtSubP analysis

AtSubP predicts an extracellular location from the signal peptide; over-annotation for an intracellularly stored myrosinase.

PMID:15539469

The vegetative vacuole proteome of Arabidopsis thaliana reveals predicted and unexpected proteins.

Vacuolar proteome of rosette leaves identifies the myrosinase gene products At5g25980 (TGG2) and At5g26000 (TGG1).

PMID:15821981

High heterogeneity within the ribosomal proteins of the Arabidopsis thaliana 80S ribosome.

80S ribosome proteomics; myrosinase among non-ribosomal co-migrating proteins.

PMID:16640593

Arabidopsis myrosinases TGG1 and TGG2 have redundant function in glucosinolate breakdown and insect defense.

tgg1 tgg2 double mutants lack leaf myrosinase activity and damage-induced aliphatic glucosinolate breakdown, and support better growth of generalist Lepidoptera; single mutants are wild-type-like (redundancy).

PMID:17951448

Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms.

Leaf peroxisome proteome includes myrosinases; authors propose an ER-derived import route and a defense role.

PMID:18431481

Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.

Large-scale chloroplast proteome; myrosinase detection likely carry-over of an abundant secretory protein.

PMID:18538804

Hydroponic isotope labelling of entire plants (HILEP) for quantitative plant proteomics; an oxidative stress case study.

HILEP quantitative proteomics of the apoplast under oxidative stress.

PMID:19433491

Myrosinases, TGG1 and TGG2, redundantly function in ABA and MeJA signaling in Arabidopsis guard cells.

tgg1-3 tgg2-1 double mutants fail to close stomata in response to ABA, MeJA and H2O2; TGG1/TGG2 act downstream of ROS and upstream of Ca2+ elevation.

PMID:21533090

Arabidopsis plasmodesmal proteome.

Plasmodesmal proteome of suspension cells, with ~35% cytoplasmic contaminants.

PMID:25293756

A proteomic strategy for global analysis of plant protein complexes.

Size-exclusion proteomics of leaf cytosolic protein complexes.

PMID:28887381

Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.

Protein correlation profiling of membrane-associated complexes.

PMID:7766881

The myrosinase gene family in Arabidopsis thaliana: gene organization, expression and evolution.

Cloning of the Arabidopsis myrosinase genes TGG1 and TGG2; in situ hybridization in leaf, sepal, petal and gynoecium.

PMID:19880612

MODIFIED VACUOLE PHENOTYPE1 is an Arabidopsis myrosinase-associated protein involved in endomembrane protein trafficking.

MVP1 interacts specifically with TGG2 (not TGG1) and is needed for correct TGG2 trafficking; mvp1 mutants mislocalize TGG2 to aggregates/ER and show increased nitrile formation on glucosinolate hydrolysis.

"glutathione S-transferase pull-down assays demonstrated that MVP1 interacted specifically with the Arabidopsis myrosinase protein, THIOGLUCOSIDE GLUCOHYDROLASE2 (TGG2), but not TGG1"

PMID:25304201

Myrosin idioblast cell fate and development are regulated by the Arabidopsis transcription factor FAMA, the auxin pathway, and vesicular trafficking.

TGG2 is expressed exclusively in myrosin idioblasts; FAMA is required for myrosin cell fate and TGG1/TGG2 expression.

"TGG1 is expressed in stomata as well as in MIs , but TGG2 is expressed exclusively in MIs ( Barth and Jander, 2006 )."

PMID:19114538

Functional proteomics of Arabidopsis thaliana guard cells uncovers new stomatal signaling pathways.

TGG2, unlike TGG1, was not detected in the guard cell proteome and is not expressed in guard cells by reporter analysis.

"Unlike TGG1, TGG2 was not found in any of the gel-based"

PMID:19703694

Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties.

Recombinant TGG1, TGG4 and TGG5 hydrolyze sinigrin and weakly hydrolyze O-beta-glucosides; used as paralog evidence for the TGG2 beta-glucosidase IBA.

"All myrosinases also displayed O-beta-glucosidase activity, although with lower efficiency compared to the myrosinase activity."